P7000

Sigma-Aldrich

胃蛋白酶 来源于猪胃粘膜

powder, ≥250 units/mg solid

别名:
胃液素 来源于猪胃, 胃蛋白酶 A
CAS号:
EC 号:
MDL编号:
eCl@ss:
42010127

形式

powder

质量水平

200

specific activity

≥250 units/mg solid

分子量

35 kDa

特色行业

Diagnostic Assay Manufacturing

UniProt登记号

运输

wet ice

储存温度

2-8°C

Gene Information

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相关类别

应用

来自Sigma的该酶已用于模拟煮熟的鳕鱼的体外胃消化。来自Sigma的该酶已用于模拟可可块和补充膳食纤维的体外胃消化。它还用于增加可提取的可溶性胶原的比例并降低从牛皮肤组织得到的胶原蛋白的免疫原性。
胃蛋白酶是一种用于消化蛋白质的肽酶,通常用于从抗体制备Fab片段。来自猪胃粘膜的胃蛋白酶已用于水解小鼠的干燥宫颈样品。 产品P7000以粉末形式提供并已用于处理来自单一猫乳腺癌的上皮细胞。
胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

包装

25, 100 g in poly bottle
1 kg in poly bottle

生化/生理作用

胃蛋白酶可水解肽键,而不是酰胺或酯键。 胃蛋白酶可在肽键的任一侧切割带有芳香酸的肽。 当含硫的氨基酸与肽键接近时,会增加对水解的敏感性。胃蛋白酶会优先在苯丙氨酸和亮氨酸的羧基侧以及谷氨酸残基的羧基侧进行切割。 切割胰岛素的β链中的Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe和Phe-Tyr键
胃蛋白酶是在胃中产生的主要蛋白水解酶。它可通过内部肽键的切割来消化蛋白质。
该酶不会在缬氨酸、丙氨酸或甘氨酸键处进行切割。Z-L-酪氨酰-L-苯丙氨酸、Z-L-谷氨酰-L-酪氨酸以及Z-L-甲硫氨酰-L-酪氨酸可被用作胃蛋白酶消化的底物。胃蛋白酶可被数种含苯丙氨酸的肽所抑制。
优先裂解:P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键

单位定义

在 37 °C,pH 2.0 条件下,一个单位的酶通过使用以血红蛋白作为基质的TCA-可溶性产物检测在每分钟可产生 0.001的 ΔA280。(终体积 = 16ml.光程 = 1cm.)

分析说明

最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。

其他说明

可访问www.sigma-aldrich.com/enzymeexplorer查看更多关于胃蛋白酶的详细信息。

象形图

Exclamation markHealth hazard

警示用语:

Danger

hazcat

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. I've seen the term "1:10000" used to define Product P7000, Pepsin from porcine gastric mucosa  (Pepsin A). What does that mean?

    A historical measurement of pepsin activity was the ratio of pepsin to coagulated egg white that the pepsin would digest. Thus 1:10,000 would refer to one part pepsin to 10,000 parts coagulated egg white.  More recent analyses use hemoglobin as a substrate, in place of coagulated egg white.

  4. Can I freeze stock solutions of Product P7000, Pepsin from porcine gastric mucosa  (Pepsin A)?

    Solutions of the enzyme at pH 4.4 should be stable at -20 °C for 2-3 months.

  5. Product P7000, Pepsin from porcine gastric mucosa  (Pepsin A), can be stored at room temperature, but other pepsins are stored in the cooler or freezer.  Why is this?

    This relates to autodigestion of pepsin.  P7000 is a relatively "crude" pepsin, where other proteins that are present would be more likely digestion targets.  With more purified pepsin, the risk of autodigestion increases, and storage at colder temperatures reduces that risk.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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实验方案
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
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相关内容
本方法可用于以血红蛋白为底物的胃蛋白酶活性测定。这是一种分光光度速率终止测定法。
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