Merck
所有图片(2)

P7626

Sigma-Aldrich

苯甲磺酰氟

≥98.5% (GC)

所有图片(2)
别名:
PMSF, 苯甲基磺酰氟, α-甲苯磺酰氟, 苄磺酰氟
Empirical Formula (Hill Notation):
C7H7FO2S
CAS号:
329-98-6
分子量:
174.19
Beilstein:
2088311
EC 号:
206-350-2
MDL编号:
MFCD00007424
PubChem化学物质编号:
24898863
NACRES:
NA.77

质量水平

300

生物来源

synthetic

测定

≥98.5% (GC)

形式

powder

mp

91-94 °C

溶解性

dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.)
H2O: unstable

SMILES string

FS(=O)(=O)Cc1ccccc1

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChI key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

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一般描述

苯甲基磺酰氟(PMSF)是一种广泛使用的丝氨酸蛋白酶抑制剂，可有效抑制胰凝乳蛋白酶、凝血酶和胰蛋白酶等酶。 PMSF通过磺化丝氨酸蛋白酶反应位点的丝氨酸残基中的羟基残基，发挥抑制作用。

应用

PMSF常用在裂解缓冲液中，通过抑制蛋白酶、避免其在细胞或组织裂解后降解，从而在蛋白分离和样品制备过程中保留目标蛋白。 PMSF在水溶性培养基中不稳定。 因此，在水溶性培养基中使用前，通常采用无水有机溶剂（例如100％乙醇或无水异丙醇）制备PMSF储备溶液。

PMSF的常见特性和优点包括：
  • 抑制丝氨酸蛋白酶，如胰蛋白酶和胰凝乳蛋白酶
  • 同时还可抑制半胱氨酸蛋白酶（通过还原硫醇可逆）和哺乳动物的乙酰胆碱酯酶
  • 有效性和毒性均不及DFP
  • 有效浓度0.1-1 mM。
  • pH 7.5时半衰期 = 1小时。
苯基甲磺酰氟已用于以下应用：
  • 细胞分级分离。
  • 在核蛋白提取中作为补充剂。
  • 胆固醇酯酶（CE）和假胆碱酯酶（PCE）抑制剂。
  • 在离心之前用于血液的收集以量化血浆ANP的水平。

包装

250 mg in glass bottle
1, 5, 25, 100 g in glass bottle

生化/生理作用

PMSF的摄入将产生与其抗胆碱酯酶作用无关的镇痛作用，并延长中枢给药的β-内啡肽的镇痛作用。

象形图

CorrosionSkull and crossbones
GHS05,GHS06

警示用语：

Danger

危险声明

H301 - H314

危险分类

Acute Tox. 3 Oral - Skin Corr. 1B

储存分类代码

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

分析证书

请输入批号搜索分析证书（COA）。

原产地证书 (CofO)

请输入批号搜索原产地证书（COO）。

Product Information Sheet

Product Information Sheet - P7626

更多文件

FT-IR Condensed Phase
FT-NMR Spectra
Spectra - ATR-IR
Spectra for FT-IR Raman
Structure Search

Quotes and Ordering

Bulk Quote-Order Product

  1. How should Product P7626, Phenylmethanesulfonyl fluoride (PMSF), solutions be prepared?

    Stock solutions of 100-200 mM can be prepared in anhydrous isopropanol or anhydrous absolute ethanol. PMSF is unstable in the presence of water.

  2. How should stock solutions of Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), be stored?

    A 200 mM solution in dry solvent is stable for at least 9 months when stored at 2-8°C.

  3. What does Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), inhibit?

    PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases.

  4. What is the effective amount of Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), to be used?

    The effective concentration range is 0.1-1 mM. A final concentration of 5 mM was used for proteinase K inhibition.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

C Pinsky et al.
Life sciences, 31(12-13), 1193-1196 (1982-09-20)
Intraperitoneal (IP) injection of the serine proteinase inhibitor phenylmethylsulfonyl fluoride (PMSF) produced dose-dependent analgesia in Sprague-Dawley rats. AD50 was 2.9 +/- 1.4 (S.E.) mg kg-1, the analgesia was antagonized by naloxone but unaffected by atropine. PMSF significantly enhanced the analgesic
Salivary esterase activity and its association with the biodegradation of dental composites.
Finer Y and Santerre JP.
Journal of Dental Research, 83(1), 22-26 (2004)
Estrogen receptors activate atrial natriuretic peptide in the rat heart.
Jankowski M, et al.
Proceedings of the National Academy of Sciences of the USA, 98(20), 11765-11770 (2001)
Lise Boussemart et al.
Nature, 513(7516), 105-109 (2014-08-01)
In BRAF(V600)-mutant tumours, most mechanisms of resistance to drugs that target the BRAF and/or MEK kinases rely on reactivation of the RAS-RAF-MEK-ERK mitogen-activated protein kinase (MAPK) signal transduction pathway, on activation of the alternative, PI(3)K-AKT-mTOR, pathway (which is ERK independent)
Rui Chen et al.
The Journal of biological chemistry, 287(36), 30800-30811 (2012-07-19)
Hypoxia-inducible factors (HIFs) are oxygen-sensitive transcription factors. HIF-1α plays a prominent role in hypoxic gene induction. HIF-2α target genes are more restricted but include erythropoietin (Epo), one of the most highly hypoxia-inducible genes in mammals. We previously reported that HIF-2α
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