Merck
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P8170

Sigma-Aldrich

Protein G−Peroxidase from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

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别名:
Protein G−HRP from Streptococcus sp.
MDL编号:
MFCD00165575
NACRES:
NA.46

重组

expressed in unspecified host

质量水平

200

偶联物

peroxidase conjugate

形式

lyophilized powder

technique(s)

direct ELISA: 1:100,000 using human IgG

储存温度

−20°C

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P8170

Protein G−Peroxidase from Streptococcus sp.

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P8045

Protein G-Biotin from Streptococcus sp.

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P8651

蛋白A-过氧物酶 来源于金黄色酿脓葡萄球菌/辣根

Millipore

Millipore

P3296

蛋白G琼脂糖凝胶™，快速流动

conjugate

peroxidase conjugate

conjugate

biotin conjugate

conjugate

peroxidase conjugate

conjugate

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

aqueous ethanol suspension

technique(s)

direct ELISA: 1:100,000 using human IgG

technique(s)

direct ELISA: 1:70,000 using human IgG

technique(s)

direct ELISA: 1:80,000 using human IgG

technique(s)

affinity chromatography: suitable

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

一般描述

Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains. Peroxidase from Streptococcus sp. is used to maintain the H2O2 homeostasis in cells. It aids the regulation of ABC Mn2+-permease complex (psaBCA) genes.

特异性

The product binds IgG for most mammalian species (excluding feline) and can be used in ELISA or immunohistochemical formats. Protein was determined to be at 250-350ug per 250ug vial by UV absorbance. Reactive to chicken IgG as well.

应用

Protein G- Peroxidase from Streptococcus sp. has been used in indirect ELISA and immunoblotting.

制备说明

Labeled with peroxidase type VI by a modification of the procedure of O′Sullivan, M.S., et al., FEBS Lett., 95, 311 (1978) which favors low molecular weight conjugates. Conjugate is purified by gel filtration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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示例

T1503
货号
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25G
包装规格/数量

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实验方案

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

以过氧化物酶 (EC 1.11.1.7) 2,2'-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）为基质的酶分析

建立以 2,2'-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）为基质的过氧化物酶测定方法。

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