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P8340

Sigma-Aldrich

Protease Inhibitor Cocktail

for use with mammalian cell and tissue extracts, DMSO solution

MDL编号:
NACRES:
NA.77

质量水平

300

生物来源

bovine lung

形式

DMSO solution

储存温度

−20°C

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特异性

抑制丝氨酸、半胱氨酸、酸性蛋白酶和氨基肽酶。

应用

蛋白酶抑制剂混合物已用于-
  • 在GST(谷胱甘肽S-转移酶)沉淀试验中作为GFP(绿色荧光蛋白)-亨廷顿转染的HEK 293细胞超声过程中的一种缓冲液成分
  • 作为裂解缓冲液中的一种成分
  • 放射免疫沉淀检测(RIPA)缓冲液中一种成分

包装

1, 5 mL in serum bottle

生化/生理作用

该混合物含有独立的成分,包括104 mM的AEBSF、80 μM的抑肽酶、4 mM的乌苯美司、1.4 mM的E-64、2mM的亮抑酶肽和1.5 mM的抑肽素A。每种成分都具有特异的抑制属性。 AEBSF和抑肽酶可抑制丝氨酸蛋白酶,包括胰蛋白酶、胰凝乳蛋白酶和纤溶酶等。 乌苯美司可抑制抑制氨肽酶。 E-64可针对半胱氨酸蛋白酶起作用。 亮抑酶肽对于丝氨酸和半胱氨酸蛋白酶都可起作用。 抑肽素A可抑制酸性蛋白酶。

组分

AEBSF
抑肽酶
乌苯美司
E-64
亮抑酶肽
抑肽素A

注意

该混合物应当被储存在-20 °C。

制备说明

一毫升的该混合物溶液推荐用于对来自20 g牛肝的100 mL裂解物或来自CHO细胞的10 mL裂解物中发现的内源酶进行抑制。 该产品是以DMSO溶液形式提供的。

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(F)

188.6 °F - closed cup

闪点(C)

87 °C - closed cup

分析证书

原产地证书 (CofO)

  1. Is there anything in Product P8340, Protease Inhibitor Cocktail, that will cause downstream problems with RT-PCR?

    While the product should not cause any downstream problems with RT-PCR, we would advise to clean up the reaction with either DMN10 (GenElute Direct mRNA Miniprep Kit) or T9424 (TRI Reagent®) for  purification.

  2. Can Product P8340, Protease Inhibitor Cocktail, be used with RIPA buffer and how much should be used?

    Yes, this product can be used with RIPA buffer at 100 μl/ml concentration.

  3. How much Product P8340, Protease Inhibitor Cocktail, should I use?

    One mL of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver or 10 mL of cell lysate obtained from CHO cells at a cell density of 100 million (108) cells per mL. CHO cells were grown on DMEM with 10% FCS (heat inactivated).  Note: Not all lysates contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

  4. What is the concentration of inhibitors in Product P8340, Protease Inhibitor Cocktail?

    The concentration of inhibitors in this product is the following:AEBSF, 104 mMAprotinin, 0.085 mMBestatin, 4 mME-64, 1.4 mMLeupeptin, 2 mMPepstain A, 1.5 mM.

  5. Does Product P8340, Protease Inhibitor Cocktail, contain any chelators or EDTA?

    This product does not contain any metal chelators.

  6. Can Product P8340, Protease Inhibitor Cocktail, be used with Ezview Protein A and Anti FLAG M2 affinity gels.

    Yes, this product can be used with both Ezview Protein A and Anti FLAG M2 affinity gels.

  7. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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