Merck
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P8340

Sigma-Aldrich

Protease Inhibitor Cocktail

for use with mammalian cell and tissue extracts, DMSO solution

All Photos(1)
MDL编号:
MFCD00677817
NACRES:
NA.77

生物来源

bovine lung

质量水平

300

形式

DMSO solution

储存温度

−20°C

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biological source

bovine lung

biological source

-

biological source

-

biological source

-

form

DMSO solution

form

DMSO solution

form

DMSO solution

form

DMSO solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

300

一般描述

蛋白酶 抑制剂混合液用于DMSO溶液中的哺乳动物细胞和组织提取物。 它含有六种单独的成分，用于抑制丝氨酸、半胱氨酸、 酸性蛋白酶和氨基肽酶。

特异性

抑制丝氨酸、半胱氨酸、酸性蛋白酶和氨基肽酶。

应用

非常适合用作在超声、裂解缓冲、和RIPA缓冲中的缓冲成分

抵御细胞和组织提取物的内源性酶活性

生化/生理作用

该混合物含有独立的成分，包括104 mM的AEBSF、80 μM的抑肽酶、4 mM的乌苯美司、1.4 mM的E-64、2mM的亮抑酶肽和1.5 mM的抑肽素A。每种成分都具有特异的抑制属性。 AEBSF和抑肽酶可抑制丝氨酸蛋白酶，包括胰蛋白酶、胰凝乳蛋白酶和纤溶酶等。 乌苯美司可抑制抑制氨肽酶。 E-64可针对半胱氨酸蛋白酶起作用。 亮抑酶肽对于丝氨酸和半胱氨酸蛋白酶都可起作用。 抑肽素A可抑制酸性蛋白酶。

特点和优势

广谱抑制：抑制多种类型的蛋白酶和氨基肽酶

稳定储存：在DMSO中作为溶液供应，允许在-20°C下长期储存

性能恒定：即用型解决方案

组分

AEBSF
抑肽酶
乌苯美司
E-64
亮抑酶肽
抑肽素A

注意

该混合物应当被储存在-20 °C。

制备说明

一毫升的该混合物溶液推荐用于对来自20 g牛肝的100 mL裂解物或来自CHO细胞的10 mL裂解物中发现的内源酶进行抑制。 该产品是以DMSO溶液形式提供的。

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H315 - H319

危险分类

Eye Irrit. 2 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(F)

188.6 °F - closed cup

闪点(C)

87 °C - closed cup

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