PC0100

Sigma-Aldrich

Protease Colorimetric Detection Kit

储存温度

2-8°C

应用

The Protease Colorimetric Detection Kit is designed for the accurate quantitation of protease activity. The method is based on the cleavage of casein into trichloroacetic acid-soluble peptides containing tyrosine and tryptophan residues. These peptides react with Folin-Ciocalteu reagent to give a characteristic blue color that can be measured at 660 nm.

适用性

Detection limit: 0.1 unit/ml (approx. 2 μg) trypsin with a 10 minute incubation time.

联系

For greater sensitivity or for detection of trace protease activity, the Protease Fluorescent Detection kit (PF0100) is recommended.

数量

Supplied with sufficient reagents to perform 200 one mL assays.

分析说明

This kit has been tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

仅试剂盒组分

产品编号
说明

  • Protease detection substrate (casein) 20 x 10

  • Folin & Ciocalteu's phenol reagent 10 mL

  • Sodium carbonate solution 125 mL

  • Enzyme diluent 125 mL

  • Tyrosine standard solution 1 mL

  • Protease from Bacillus polymyxa 1 g

  • Trichloroacetic acid (TCA) solution 5 mL

分析证书

原产地证书 (CofO)

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门