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  • PCRISPR002
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PCRISPR002

Sigma-Aldrich

Mouse CRISPR Activation Caprano Library

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CRISPRILIB

Human Whole Genome and Long Non Coding CRISPRi Library Kit

包装

pkg of 10 vials (5x200µL aliquots of each component)

浓度

≥5x108 VP/ml (via p24 Assay)

application(s)

CRISPR

运输

dry ice

储存温度

−70°C

一般描述

The mouse CRISPR activation library (Caprano) activates over 22,000 mouse genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018).

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

应用

  • Functional Genomics/Target Validation
  • Focused forward genetic activation screening
  • Validated positive and negative controls

特点和优势

  • Focus on your research, and we will generate your lentivirus screening library
  • Compatible with SAMHELPERV for consistent effector expression across a wide variety of cell lines dCas9-VP64-Blasticidin MS2-P65-HSF1-Hygromycin
  • Targeting Genes22,774 (Set A), 22,658 (Set B)
  • Compact Libary with gRNAs 67,187 (Set A), 66,889 (Set B)
  • Built-in Controls 500, unique non-targeting controls are in each of the two half-libraries (Set A and Set B)

组分

2 Subpools with a minimum concentration of 5x108 VP/mL(via p24 assay)
PCRISPR002A - Mouse CRISPR Activation Caprano Library Set A
PCRISPR002B - Mouse CRISPR Activation Caprano Library Set B

原理

The most effective CRISPRa system uses the CRISPR Synergistic Activation Mediator (SAM) complex. This platform combines VP64-dCas9 with a modified guide. In addition, the gRNA contains two aptamers that recruit additional co-activators, p65 and HSF1, enhancing target gene activation.

CRISPRa can also serve as a complementary approach to loss-of-function (LOF) genetic screens by enriching for a different set of genes responsible for a phenotype CRISPRa screens show little-to-no off-target activity and can be used to study genes that are difficult to characterize using only LOF approaches because of their high copy number.

法律信息

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H317

危险分类

Skin Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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