Merck
All Photos(2)

PHG0001

SAFC

HEPES

Synonym(s):
4-(2-羟乙基)哌嗪-1-乙磺酸, N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.21

生物来源

synthetic

质量水平

400

形式

powder

technique(s)

cell culture | mammalian: suitable

杂质

Endotoxin, microbial, and trace metals; tested

pH值(酸碱度)

4.7-5.3 (, )

useful pH range

6.8-8.2

pKa (25 °C)

7.5

适用性

suitable for manufacturing use

异质活性

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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包装

100 g in poly bottle
5, 10, 25, 50 kg in poly drum

应用

HEPES是一种生物缓冲液,通常被称为“好”缓冲液。它是最常用的生物缓冲液,因为HEPES的pKa为7.31,与生理pH值7.36相对应。HEPES被认为是对培养细胞系无毒的一种物质,高度可溶于水并可为溶液提供高澄清度。

HEPES用于细胞培养基、生物制药缓冲制剂(上游和下游)以及诊断试剂。基于HEPES的缓冲液被用于纯化抗体、生物活性物质、蛋白质和生长因子的生物过程。

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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