Merck
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PIR00

Sigma-Aldrich

Inhibitor Resistant Genotyping PCR ReadyMix

for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

Synonym(s):
Inhibitor Resistant Genotyping PCR ReadyMix
NACRES:
NA.55

质量水平

200

形式

liquid

用途

sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions

特点

Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart

储存条件

protect from light

technique(s)

qPCR: suitable

颜色

light blue

input

crude DNA

相容性

Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad MJ Chromo4
Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
Bio-Rad MiniOpticon
Cepheid SmartCycler
Eppendorf® Mastercycler ep realplex
Eppendorf® Mastercycler ep realplex2 S
Illumina Eco qPCR
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
Roche LightCycler 480

detection method

probe-based

运输

dry ice

储存温度

−20°C

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一般描述

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use master mix for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.

A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

应用

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart® One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye.
Inhibitor Resistant Genotyping PCR ReadyMix has been used as a component of the reaction mixture for detection of leucoanthocyanidin reductase(PaLAR3) alleles in a genotyping assay and multiplex PCR for amplification of microsatellite.

特点和优势

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required
  • Compatible with both fast and conventional PCR cycling protocols

组分

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

其他说明

Compatible instruments include:
  • Bio-Rad® CFX384
  • Bio-Rad CFX96
  • Bio-Rad MiniOpticon
  • Bio-Rad/MJ Chromo4
  • Bio-Rad/MJ Opticon 2
  • Bio-Rad/MJ Opticon
  • Cepheid SmartCycler®
  • Eppendorf Mastercycler® ep realplex
  • Eppendorf Mastercycler ep realplex2 s
  • Illumina Eco qPCR
  • Qiagen/Corbett Rotor-Gene® 3000
  • Qiagen/Corbett Rotor-Gene 6000
  • Qiagen/Corbett Rotor-Gene Q
  • Roche LightCycler® 480

法律信息

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Chromo4 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf AG
KiCqStart is a registered trademark of Qiagen Beverly Inc.
LightCycler is a registered trademark of Roche
Mastercycler is a registered trademark of Eppendorf AG
MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.
Opticon is a trademark of Bio-Rad Laboratories, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
SmartCycler is a registered trademark of Cepheid, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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