PROTSIL1

Sigma-Aldrich

ProteoSilver 银染试剂盒

别名:
银染试剂盒

用途

 kit sufficient for 25 mini-gels (10 × 10 cm)

质量水平

200

应用

protein staining: suitable

一般描述

ProteoSilver具有便于使用的包装,稳定的性能,极高的灵敏度,适合任何蛋白研究科学家使用。该试剂盒含有制备好的银染试剂,并配有如何获得最佳结果的详细说明。ProteoSilver的检出限为0.1 ng/mm2的蛋白质(BSA),本底极低,可对低丰度蛋白进行出色的检测。

应用

ProteoSilver 银染试剂盒已被用于凝胶染色以对蛋白进行显色。

特点和优势

  • 经预混和预称重 的溶液可减少对每一成分单独进行采购及制备所花费的时间和成本。
  • 经优化的实验方案 有助于确定获得最佳结果所需的条件。
  • 其高度的灵敏度及极低的本底 可确保对低丰度蛋白的检测并从其他蛋白中分辨出来。
  • 兼容于MALDI 感兴趣蛋白点可通过质谱进行进一步的定性分析。
  • 室温稳定性使得其保存容易而且方便。

法律信息

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

警示用语:

Danger

hazcat

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Muta. 2 - Ox. Liq. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 2 - STOT SE 3

靶器官

Eyes, Respiratory system

storage_class_code

5.1B - Oxidizing hazardous materials

WGK Germany

WGK 3

闪点(F)

132.8 °F - closed cup

闪点(C)

56 °C - closed cup

分析证书

原产地证书 (CofO)

Yong-Feng Han et al.
The Plant cell, 28(5), 1215-1229 (2016-04-27)
The mechanism by which MORPHEUS' MOLECULE1 (MOM1) contributes to transcriptional gene silencing has remained elusive since the gene was first identified and characterized. Here, we report that two Arabidopsis thaliana PIAS (PROTEIN INHIBITOR OF ACTIVATED STAT)-type SUMO E3 ligase-like proteins...
Jean-Paul Lasserre et al.
Electrophoresis, 27(16), 3306-3321 (2006-07-22)
Study of the complexome - all the protein complexes of the cell - is essential for a better understanding and more global vision of cell function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic and membrane protein complexes of...
Yongyu Huang et al.
The Plant journal : for cell and molecular biology, 96(4), 716-733 (2018-08-14)
Inflorescence branching is a key agronomic trait determining rice yield. The primary branch of the ancestral wild rice (Oryza rufipogon Griff.) bears few grains, due to minimal secondary branching. By contrast, Oryza sativa cultivars have been selected to produce large...
Jing Tian et al.
eLife, 7 (2018-06-29)
In obesity, elevated insulin causes fatty liver by activating the gene encoding SREBP-1c, a transcription factor that enhances fatty acid synthesis. Two transcription factors, LXRα and C/EBPβ, are necessary but not sufficient for insulin induction of hepatic SREBP-1c mRNA. Here...
Yong-Feng Han et al.
Cell research, 24(12), 1445-1465 (2014-11-26)
The SU(VAR)3-9-like histone methyltransferases usually catalyze repressive histone H3K9 methylation and are involved in transcriptional gene silencing in eukaryotic organisms. We identified a putative SU(VAR)3-9-like histone methyltransferase SUVR2 by a forward genetic screen and demonstrated that it is involved in...
技术文章
Bis-Tris gels and buffers for superior protein resolution compared to traditional tris-glycine gels.
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实验方案
Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits
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