PROTSIL2

Sigma-Aldrich

ProteoSilver Plus 银染试剂盒

NACRES:
NA.32

用途

 kit sufficient for 25 mini-gels (10 × 10 cm)

质量水平

200

应用

protein staining: suitable

储存温度

20-25°C

一般描述

ProteoSilver Plus银染试剂盒具有方便的包装、极稳定的性能和高灵敏度,是所有蛋白质研究科学家的理想选择。 与ProteoSilver(PROTSIL1)相似,该试剂盒含有制备好的银染试剂,并配有如何获得最佳结果的详细说明。 ProteoSilver Plus的检出限为0.1 ng/mm2 的蛋白质(BSA),本底极低,可对低丰度蛋白进行出色的检测。
ProteoSilver Plus也可用于MALDI-MS检测,其不含会使赖氨酸残基发生交联的戊二醛,该成分会导致质谱结果失准。ProteoSilver Plus银染试剂盒另外含有两种可使切离蛋白点脱色的试剂,适于希望通过胰蛋白酶消化和MALDI-MS分析来进一步研究蛋白特性的研究人员。

应用

ProteoSilver® 银染试剂盒已可用于凝胶染色,以便观察显色的蛋白质。该试剂盒提供的脱色剂可用于使感兴趣的蛋白脱色,以便进行质谱分析。

特点和优势

  • 经预混和预称重的溶液可减少对每一成分单独进行采购及制备所花费的时间和成本。
  • 经优化的实验方案 有助于确定获得最佳结果所需的条件。
  • 其高度的灵敏度及极低的本底可确保对低丰度蛋白的检测并从其他蛋白中分辨出来。
  • 兼容于MALDI感兴趣蛋白点可通过质谱进行进一步的定性分析。
  • 室温稳定性 使得其保存容易而且方便。

法律信息

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
Sigma-Aldrich is a registered trademark of Sigma-Aldrich Co. LLC

警示用语:

Danger

hazcat

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 2 - STOT SE 3

靶器官

Eyes, Respiratory system

补充剂危害

EUH032

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 3

闪点(F)

149.0 °F - closed cup

闪点(C)

65 °C - closed cup

K P Aggarwal et al.
International braz j urol : official journal of the Brazilian Society of Urology, 39(1), 128-136 (2013-03-16)
Proteins constitute a major portion of the organic matrix of human calcium oxalate (CaOx) renal stones and the matrix is considered to be important in stone formation and growth. The present study evaluates the effect of these proteins on oxalate...
Priyadarshini Pathak et al.
International braz j urol : official journal of the Brazilian Society of Urology, 36(5), 621-628 (2010-11-04)
Investigate the activity of high and low molecular weight biomolecules present in the matrix of human calcium oxalate (CaOx) stones not only on the initial mineral phase formation of calcium and phosphate (CaP) but also on its growth and demineralization...
2D map of proteins from human renal stone matrix and evaluation of their effect on oxalate induced renal tubular epithelial cell injury.
Aggarwal KP, et al.
International Brazilian Journal of Urology : Official Journal of the Brazilian Society of Urology, 39, 128-136 (2013)
Xiancai Ma et al.
eLife, 8 (2019-01-18)
Comprehensively elucidating the molecular mechanisms of human immunodeficiency virus type 1 (HIV-1) latency is a priority to achieve a functional cure. As current 'shock' agents failed to efficiently reactivate the latent reservoir, it is important to discover new targets for...
Lina Fassi Fehri et al.
Antimicrobial agents and chemotherapy, 49(10), 4154-4165 (2005-09-29)
Antimicrobial peptides are widely distributed in nature, and in vertebrates, they play a key function in the innate immune defense system. It is generally agreed that these molecules may provide new antibiotics with therapeutic value. However, there are still many...

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