所有图片(1)

R5125

Sigma-Aldrich

核糖核酸酶A 来源于牛胰腺

Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

别名:
核糖核酸 3′-嘧啶寡核苷酸水解酶, RNAsea, RNase A, 核糖核酸酶 I, 胰核糖核酸酶
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

质量水平

300

类型

Type III-A

测定

≥85% RNase A basis (SDS-PAGE)

形式

lyophilized powder

specific activity

85-140 Kunitz units/mg protein

分子量

~13,700

杂质

salt, essentially free

特色行业

Diagnostic Assay Manufacturing

异质活性

protease, essentially free

储存温度

−20°C

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一般描述

RNase A(核糖核酸酶A)是一种内切核糖核酸酶,在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端(例如,pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG)。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同,并非糖蛋白。核糖核酸酶不会水解DNA,因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外,RNase也被DNA竞争性抑制。

应用

  • RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
  • RNase A还用于RNA序列分析和保护测定。
  • RNase A已用作计算辅助药物设计的工具。
  • RNase A为RNA序列分析提供支持。
  • RNase A水解蛋白质样品中的RNA。
  • RNase A为DNA纯化提供支持。
核糖核酸酶A被用于去除DNA质粒制品蛋白质样品中的RNA。 核糖核酸酶A被用于RNA酶保护测定、去除非特异性结合的RNA、分析RNA序列、水解蛋白质样品包含的RNA以及DNA纯化。来自牛胰腺的核糖核酸酶A已可用于评估蛋白质的镍依赖性氧化交联。 来自牛胰腺的核糖核酸酶A还被用于研究蛋白质与DNA的非特异性结合的平衡常数。

包装

1 g in glass bottle
25, 50, 100, 250, 500 mg in glass bottle

生化/生理作用

核糖核酸酶A是一种内切核糖核酸酶,可在嘧啶核苷酸后切割单链RNA。它在3'磷酸基末端进行攻击。核糖核酸酶不会水解DNA,因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNA酶还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。

特点和优势

我们高度稳定的核糖核酸酶A——RNase A,适合于RNA去除、RNA测序和DNA纯化。

制备说明

盐组分分离和色谱纯化。

分析说明

蛋白测定方法:E.

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

E S Jenuwine et al.
Analytical biochemistry, 242(2), 228-233 (1996-11-15)
Quantitative zonal DNA affinity chromatography may be used to determine accurate equilibrium constants for the binding of proteins nonspecifically to DNA. Zonal quantitative affinity chromatography has not previously been applied to the determination of binding constants of proteins to DNA...
Thomas Blasi et al.
Nature communications, 7, 10256-10256 (2016-01-08)
Imaging flow cytometry combines the high-throughput capabilities of conventional flow cytometry with single-cell imaging. Here we demonstrate label-free prediction of DNA content and quantification of the mitotic cell cycle phases by applying supervised machine learning to morphological features extracted from...
G Gill et al.
Chemical research in toxicology, 10(3), 302-309 (1997-03-01)
A model protein, ribonuclease A (bovine pancreas), was examined for its ability to coordinate Ni2+ and promote selective oxidation. In the presence of a peracid such as monopersulfate, HSO5-, nickel induced the monomeric RNase A to form dimers, trimers, tetramers...
S B delCardayré et al.
Protein engineering, 8(3), 261-273 (1995-03-01)
Bovine pancreatic ribonuclease A (RNase A) has been the object of much landmark work in biological chemistry. Yet the application of the techniques of protein engineering to RNase A has been limited by problems inherent in the isolation and heterologous...
Aida Muslimovic et al.
Nature protocols, 3(7), 1187-1193 (2008-07-05)
Phosphorylation of histone protein H2AX on serine 139 (gamma-H2AX) occurs at sites flanking DNA double-stranded breaks (DSBs) and can provide a measure of the number of DSBs within a cell. We describe a flow cytometry-based method optimized to measure gamma-H2AX...

实验方案

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

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