Merck

R7388

Sigma-Aldrich

RPMI-1640 培养基

Modified, with 20 mM HEPES and L-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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别名:
Roswell Park Memorial Institute 1640 medium
MDL编号:
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

pH值(酸碱度)

>7.2

组分

HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes

运输

ambient

储存温度

2-8°C

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form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

components

HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

一般描述

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
RPMI-1640培养基配方基于RPMI-1630系列培养基,利用碳酸氢盐缓冲系统和氨基酸和维生素数量的改变。用于人正常白细胞和肿瘤白细胞的培养。RPMI-1640还支持各种类型的细胞培养物的生长,如72小时植物血凝素(PHA)刺激试验中的新鲜人淋巴细胞。

应用

RPMI-1640培养基已用于培养:
  • 人永生角化细胞、体外转染试验
  • 细胞系和人T细胞母细胞,监控自噬流量
  • 小猪外周血单核细胞(PBMC)

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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