Merck
  • R7757
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R7757

Sigma-Aldrich

红细胞裂解缓冲液Hybri-Max

liquid, sterile-filtered, suitable for hybridoma

Synonym(s):
RBC裂解缓冲液
NACRES:
NA.75

无菌性

sterile-filtered

质量水平

形式

liquid

technique(s)

cell culture | hybridoma: suitable

杂质

endotoxin, tested

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This Item
H8889A7422H0137
sterility

sterile-filtered

sterility

sterile-filtered

sterility

γ-irradiated

sterility

γ-irradiated

form

liquid

form

liquid

form

lyophilized powder

form

lyophilized powder

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable, single cell analysis: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

Quality Level

400

Quality Level

400

Quality Level

200

Quality Level

200

RBC lysing buffer, RBC solubilization buffer

density gradient, gradient centrifugation, lymphocyte purification reagent

Sigma-Aldrich A7422, AT Media Supplement (50×) Hybri-Maxγ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma, adenine-thymidine cocktail, AT media hybridoma selection, fusion media

hypoxanthine-thymidine supplement, hybridoma cloning, hybridoma selection

应用

建议在细胞颗粒中加入1 mL缓冲液(细胞颗粒=1个脾脏或1-2亿个细胞)。 轻轻混合1分钟。 用15-20mL培养基活盐溶液稀释该缓冲液。 在250-500 × g 离心7分钟并弃去上清。 细胞可被稀释并准备计数或融合。 如果裂解不完全,可重复1-4步。

生化/生理作用

红细胞裂解缓冲液已被开发用于杂交瘤实验中,用于在融合前从小鼠脾细胞悬液中去除红细胞。 在需要从细胞悬液(如全血)中去除红细胞的体系中,它也很有用。

组分

含8.3 g/L 氯化铵的0.01 M Tris-HCl 缓冲液。

其他说明

注:本品用于去除小鼠红细胞。 本品可能不适用于其他动物红细胞的裂解。 该产品在小鼠脾细胞以外的任何应用场合的适用性必须由研究人员确定。

法律信息

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(F)

Not applicable

闪点(C)

Not applicable


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