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R8758

Sigma-Aldrich

RPMI-1640 培养基

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

MDL编号:
NACRES:
NA.75

质量水平

400

无菌性

sterile-filtered

形式

liquid

应用

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

sodium pyruvate: no
L-glutamine: yes
phenol red: yes
HEPES: no
NaHCO3: yes

运输

ambient

储存温度

2-8°C

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一般描述

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。通过对McCoy′s 5A培养基进行修改,它被配制用于在悬浮培养中支持淋巴母细胞,但一直以来它也被证明可以支持各种不同锚定依赖的细胞。

应用

RPMI-1640培养基已被用作维持细胞系的培养基并作为不同细胞的培养基。
RPMI 1640 培养基由 Moore 及其同事于 1966 年在 Roswell Park Memorial Institute 开发。它是 McCoy′s 5A 培养基的改良型,在悬浮培养中可支持淋巴母细胞样细胞,但也证实可支持众多锚定依赖性细胞。最初设计为需要补充血清,但在无血清条件下,RPMI 1640 也表现出可支持多种细胞系的生长。它还广泛用于细胞融合和杂交细胞的生长。

联系

需要FBS? 我们可以协助您的血清计划。 请访问血清计划者了解更多信息。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Does Product R8758, RPMI-1640 Medium, contain glucose?

    RPMI contains glucose at 2 g/L.  The complete formulation of RPMI and other classical media formulations can be found on the Cell Culture Web Page.

  3. Can I add the glutamine dipeptide to Product R8758, RPMI-1640 Medium?

    This formulation of RPMI already contains L-glutamine at 0.3 g/L.  It does not require further supplementation with L-glutamine or the L-glutamine dipeptide (Product No. G8541).

  4. Does Product R8758, RPMI-1640 Medium, contain HEPES?

    There is no HEPES in this formulation of RPMI.  We do offer RPMI containing HEPES as Product No's. R5886 and R4130. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.

  5. Can Product R8758, RPMI-1640 Medium, be frozen?

    We do not recommend freezing cell culture media. A precipitate may form upon thawing.  Also, this can cause a loss of amino acids, which may yield low cell growth.

  6. Does Product R8758, RPMI-1640 Medium, contain phenol red?

    This media contains phenol red.  The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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