Merck
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RAB0507

Sigma-Aldrich

人VEGF-A ELISA试剂盒

for serum, plasma, cell culture supernatants and urine

Synonym(s):
VEGF-A
NACRES:
NA.32

species reactivity

human

包装

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

detection method

colorimetric

运输

wet ice

储存温度

−20°C

Gene Information

human ... VEGFA(7422)

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RAB0195RAB0328RAB1095
species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma
sample type urine

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

input

sample type plasma
sample type serum sample(s)
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 12.29-3000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 70 pg/mL
standard curve range: 34.3-25000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.4 ng/ml

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

一般描述

人VEGF ELISA(酶联免疫吸附测定)试剂盒采用体外酶联免疫吸附测定法,定量测定人血清、血浆、细胞培养物上清液和尿液中的VEGF含量。

免疫原

重组人VEGF-A

应用

人 VEGF-A ELISA 试剂盒已用于定量测定:
  • 人肝癌细胞系PLC-PRF-5中的VEGF,
  • 去细胞化脑膜支架(decellularized meningeal scaffold,DMS)中的残留细胞因子
  • 人牙周膜(hPDL)成纤维细胞

仅供研究使用。不可用于诊断过程。
人VEGF(血管内皮生长因子)ELISA试剂盒已被用于测定与先兆子痫和健康孕妇血浆一起孵育的人脐带血管内皮细胞(HUVEC)中的VEGFA水平。

生化/生理作用

VEGF(血管内皮生长因子)是一种有效的血管生成细胞因子。它可刺激内皮细胞的增殖和迁移。VEGF通过增强囊泡-液泡细胞器(VVO)功能诱导微血管通透性。VEGF在正常血管生成和病理性血管生成中都发挥着重要作用。大量证据表明VEGF涉及肿瘤转移和眼内新生血管综合征的诱导。VEGF表达增加导致视网膜血管生成,因此,使用可溶性VEGF受体嵌合蛋白抑制VEGF可以被认为是一种治疗缺血性视网膜疾病的潜在方法。VEGF在C (-634) G的多态性与2型糖尿病的糖尿病视网膜病变相关。VEGF表达的改变也与类风湿性关节炎、格雷夫斯病、牛皮癣、心血管疾病和外周肢体疾病相关。

其他说明

本产品提供样本检验报告。
请在批号对应的文本框中输入样品 一词。

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

象形图

Corrosion

警示用语:

Warning

危险声明

预防措施声明

危险分类

Met. Corr. 1

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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