Merck
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S1638

Millipore

链霉亲和素-琼脂糖 来源于阿维丁链霉菌

buffered aqueous suspension

Synonym(s):
streptavidin agarose beads, streptavidin agarose resin
MDL编号:
NACRES:
NA.56

形式

buffered aqueous suspension

质量水平

标记范围

≥1 mg per mL

基质

4% beaded agarose

基质活化

cyanogen bromide

基质附着

amino

基质隔离区

7 atoms

容量

≥15 μg/mL binding capacity (biotin)

储存温度

2-8°C

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This Item
S5512S6402S3402
form

buffered aqueous suspension

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

extent of labeling

≥1 mg per mL

extent of labeling

-

extent of labeling

3-9 mol fluorochrome per mol protein

extent of labeling

-

matrix

4% beaded agarose

matrix

-

matrix

-

matrix

-

matrix activation

cyanogen bromide

matrix activation

-

matrix activation

-

matrix activation

-

应用

来自链霉菌的链霉亲和−琼脂糖已用于:
  • 在质膜转化生长因子β(TGFβ)受体II (TβRII)定量和Tβ
  • RII内化过程中pull-down生物素化的细胞表面蛋白
  • 生物素化miRNA pull-down实验;作为免疫沉淀的二级抗体

链亲和素琼脂糖用于蛋白质层析,亲和层析和重组蛋白表达和分析。链亲和素琼脂糖可以被用来研究烟草蚀纹病毒蛋白酶切割融合蛋白的定向固定。链亲和素琼脂糖被用来研发从天然植物提取物中筛选三重DNA结合物。
用于纯化含有蛋白质或DNA结合蛋白质的生物素

生化/生理作用

链霉亲和素是一种同型四聚体蛋白,分离自亲和素链霉菌中,与亲和素一样,对生物素具有很高的亲和力。链霉亲和素为轻度阴离子(pI ~ 5-6),非糖基化。与蛋清亲和素相比,这些特性使其非特异性结合力相对较低。链霉亲和素也比亲和素更不易被氯化胍分解成亚基。链霉亲和素-琼脂糖可用于固定或分离各种生物素化大分子和络合物(蛋白质、抗体、凝集素、核酸、受体和配体)。链霉亲和素-生物素相互作用需要苛刻的条件才能释放生物素化大分子。这一特性使得链霉亲和素-琼脂糖广泛用于各种亲和纯化应用。

外形

在pH7.2、含有0.05M NACl和0.02%叠氮化钠的0.01 M磷酸钠缓冲液中复溶

储存分类代码

10 - Combustible liquids

闪点(F)

Not applicable

闪点(C)

Not applicable


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