Merck

S2014

Sigma-Aldrich

Staphylococcus aureus

buffered aqueous suspension, Wood 46 strain

NACRES:
NA.81

生物来源

Staphylococcus aureus

无菌性

Not processed or packaged aseptically

形式

buffered aqueous suspension

组成

Cell suspension, ~10% wet weight/volume

储存温度

2-8°C

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P6031P7155539202
Sigma-Aldrich

Sigma-Aldrich

S2014

Staphylococcus aureus

form

buffered aqueous suspension

form

essentially salt-free, lyophilized powder

form

cell suspension

form

lyophilized solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

sterility

Not processed or packaged aseptically

sterility

-

sterility

(not processed or packaged aseptically)

sterility

-

composition

Cell suspension, ~10% wet weight/volume

composition

-

composition

-

composition

-

一般描述

Staphylococcus aureus Wood 46 is protein A deficient and spa negative. It shares 98% to 99% genome identity with S.aureus and shows a lower surface expression of cell wall-associated protein A.

应用

(Not intended for use as a starter culture.)
Staphylococcus aureus has been used:
  • to mimic infection and induce fever in Pekin duck
  • to test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larva
  • in the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedunin potassium salt

Wood 46, a non-protein A producing S. aureus strain, prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.

生化/生理作用

Staphylococcus aureus Wood 46 displays reduced virulence compared to the S. aureus. This isolate is useful in understanding protein A role in pathogenesis and virulence.

外形

Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide

制备说明

Produced in pure culture.

分析说明

This strain binds less than 10% of the rabbit IgG bound by P 7155 as assayed by a modification of the method of Kessler.

储存分类代码

6.2 - Infectious substances

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable


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其他客户在看

Manasi Balachandran et al.
Genome announcements, 5(13) (2017-04-01)
Here, we report the first complete genome sequence of the Staphylococcus aureus strain Wood 46. Wood 46 has played an important role in understanding the virulence and pathogenesis of S. aureus infections. This report will assist efforts in vaccine development against
M Marais et al.
Poultry science, 90(6), 1234-1238 (2011-05-21)
Poultry, like mammals and other birds, develop fever when exposed to compounds from gram-negative bacteria. Mammals also develop fever when exposed to the constituents of viruses or gram-positive bacteria, and the fevers stimulated by these different pathogenic classes have discrete
G Kronvall et al.
Infection and immunity, 3(1), 10-15 (1971-01-01)
Protein A of Staphylococcus aureus can be detected on cell walls of intact bacteria by use of radioactively labeled myeloma globulin. Of 156 strains of S. aureus, 141 (90%) contained protein A. None of 47 S. epidermidis strains was positive
Synthetic modification of gedunin and comparative antibacterial activity of gedunin and 7-deacetoxy-7alpha-hydroxygedunin potassium salt
Okhale SE, et al.
African Journal of Pure and Applied Chemistry, 6(14), 183-189 (2012)
S W Kessler
Journal of immunology (Baltimore, Md. : 1950), 117(5 Pt 1), 1482-1490 (1976-11-01)
Procedures are detailed for the rapid isolation of representative cell membrane antigens with protein A-bearing staphylococci as an adsorbent for IgG antibodies complexed with the antigens. Cell surface membrane proteins were radioiodinated and solubilized in nonionic detergent. Specific antisera were

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