Merck
  • S2890
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S2890

Sigma-Aldrich

链霉亲和素-碱性磷酸酶 来源于阿维丁链霉菌

lyophilized powder, ≥700 DEA units/mg protein

Synonym(s):
Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii
MDL编号:
NACRES:
NA.46

偶联物

alkaline phosphatase conjugate

质量水平

形式

lyophilized powder

specific activity

≥700 DEA units/mg protein

组成

Protein, ≥40% biuret

储存温度

−20°C

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This Item
S3762S5512S6402
form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

specific activity

≥700 DEA units/mg protein

specific activity

≥5 units/mg protein

specific activity

-

specific activity

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

alkaline phosphatase conjugate

conjugate

FITC conjugate

conjugate

peroxidase conjugate

conjugate

CY3 conjugate

composition

Protein, ≥40% biuret

composition

-

composition

Protein, ≥70% E1%/280

composition

-

一般描述

链霉亲和素是一种58 kDa蛋白质,是从抗生链霉菌纯化所得的高四聚体。它对生物素具有较高的亲和力。链霉亲和素已可与过氧化物酶或碱性磷酸酶(ALP)偶联结合。这些酶相对稳定且针对不同应用提供了广泛的适宜底物,因而常被用作蛋白偶联物。在免疫测定中,ALP与链霉亲和素偶联结合。

应用

最佳的工作稀释度应通过 1mg/mL 的储存缓冲液进行一系列的稀释后凭经验确定。
链霉亲和素碱性磷酸酶来自抗生蛋白链霉菌,已用于酶联免疫吸附斑点(ELISpot)印迹,以检测生产干扰素(IFN-γ)的细胞。

单位定义

一单位碱性磷酸酶可在pH9.8,37℃条件下每分钟水解1.0 μmole p-硝基苯磷酸。

外形

冻干粉中含有带有EPPS的海藻糖,磷酸和痕迹量氯化镁和氯化锌。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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