- S5692
Recommended Products
保质期
≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.)
质量水平
technique(s)
protein staining: suitable
荧光
λex 300,470 nm; λem 570 nm
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This Item | S4942 | RSB | G1041 |
|---|---|---|---|
| shelf life ≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.) | shelf life ≥6 mo. (when stored at room temperature and protected from light) | shelf life - | shelf life - |
| technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) protein staining: suitable |
| fluorescence λex 300,470 nm; λem 570 nm | fluorescence λex 280,450 nm; λem 610 nm | fluorescence - | fluorescence - |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
一般描述
SYPRO Orange为：
- 高灵敏度。 该染色剂可检测每minigel条带中1-2ng的蛋白质，比考马斯亮蓝或银染色更灵敏。
- 快速。 在不到1小时内完成染色。
- 简单。 电泳后，对凝胶进行染色、冲洗并拍照；不需要固定或脱色步骤
- 与标准实验室设备兼容。 染色蛋白可以用标准的300纳米紫外透照仪或激光扫描仪来观察。
- 物美价廉。 SYPRO Orange染色法较银染法便宜，并且所需时间短。
- 不同蛋白间变化小。 染料与蛋白质周围的SDS包被层相互作用，使不同类型的蛋白质之间的染色比考马斯或银染色更一致。
- 对蛋白的选择性。 SYPRO Orange检测的蛋白质小至6.5 kDa，不染色核酸或脂多糖。 它染色糖基化蛋白。
- 较宽的检测线性范围。 染色条带的荧光强度与超过3个数量级的蛋白量呈线性相关(比考马斯染色或银染色范围更广)。
应用
注意
法律信息
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(F)
188.6 °F - closed cup
闪点(C)
87 °C - closed cup
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