Merck
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S5692

Supelco

SSYPRO® Orange蛋白凝胶染色剂

Synonym(s):
SYPRO® dye, protein gel stain
EC 号:

保质期

≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.)

质量水平

technique(s)

protein staining: suitable

荧光

λex 300,470 nm; λem 570 nm

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S4942RSBG1041
shelf life

≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.)

shelf life

≥6 mo. (when stored at room temperature and protected from light)

shelf life

-

shelf life

-

technique(s)

protein staining: suitable

technique(s)

protein staining: suitable

technique(s)

protein staining: suitable

technique(s)

protein staining: suitable

fluorescence

λex 300,470 nm; λem 570 nm

fluorescence

λex 280,450 nm; λem 610 nm

fluorescence

-

fluorescence

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

一般描述

SYPRO Orange与SYPRO Red相似,但它颜色更亮一些,背景荧光略高。 对于使用激光激发凝胶扫描仪的用户,推荐使用一种基于氩离子激光的SYPRO Orange仪器。 该染料可由紫外线或宽带照明光有效激发。它不适用于印迹或IEF凝胶中蛋白的染色,并在2维凝胶染色中显示出较低的灵敏度。
SYPRO Orange为:
  • 高灵敏度。 该染色剂可检测每minigel条带中1-2ng的蛋白质,比考马斯亮蓝或银染色更灵敏。
  • 快速。 在不到1小时内完成染色。
  • 简单。 电泳后,对凝胶进行染色、冲洗并拍照;不需要固定或脱色步骤
  • 与标准实验室设备兼容。 染色蛋白可以用标准的300纳米紫外透照仪或激光扫描仪来观察。
  • 物美价廉。 SYPRO Orange染色法较银染法便宜,并且所需时间短。
  • 不同蛋白间变化小。 染料与蛋白质周围的SDS包被层相互作用,使不同类型的蛋白质之间的染色比考马斯或银染色更一致。
  • 对蛋白的选择性。 SYPRO Orange检测的蛋白质小至6.5 kDa,不染色核酸或脂多糖。 它染色糖基化蛋白。
  • 较宽的检测线性范围。 染色条带的荧光强度与超过3个数量级的蛋白量呈线性相关(比考马斯染色或银染色范围更广)。

应用

SYPRO Orange蛋白凝胶染色技术已应用于蛋白质的热变性和热转移实验。它也被用于微分扫描荧光法。

注意

SYPRO储液应在室温、4°C或20°C下干燥保存并避光。如果储存得当,这些储液可以稳定使用6个月到1年。使用缓冲液或醋酸溶液稀释后的染色试剂可以储存在非常干净、无去污剂的玻璃瓶或塑料瓶中,在4℃下避光保存至少3个月。 

法律信息

SYPRO is a registered trademark of Life Technologies

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

188.6 °F - closed cup

闪点(C)

87 °C - closed cup


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