S8445

Sigma-Aldrich

SigmaMarker

wide range, mol wt 6,500-200,000 Da

MDL编号:
NACRES:
NA.25

质量水平

200

形式

lyophilized powder

用途

sufficient for 10-20 large gels (16 × 18 cm)
sufficient for 20-30 mini-gels (10 × 10 cm)
 vial sufficient for 50-100 PhastGels

分子量

6,500-200,000 Da

储存温度

2-8°C

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相关类别

应用

SigmaMarker已被用作十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)中的蛋白质分子量标准。
SigmaMarkers设计用于Laemmli SDS-PAGE、PhastSystem (Pharmacia) 、以及Schagger和von Jagow 的SDS-PAGE(三甲基甘氨酸)。 SigmaMarkers涵盖大多数蛋白质或其亚基共有的分子量范围。宽、高和低范围分子量标准品与样品缓冲液一起冻干,便于用去离子水溶解后即可使用。该分子量标准品已经过配制,可在电泳和考马斯蓝染色后产生大致相等强度的明确条带分布。

其他说明

蛋白质(分子量)
抑肽酶,牛肺(6,500)
α -乳清蛋白,牛奶(14,200)
胰蛋白酶抑制剂,大豆(20,000)
胰蛋白酶原,牛胰腺(24,000)
碳酸酐酶,牛红细胞(29,000)
甘油醛-3-磷酸脱氢酶,兔肌肉(36,000)
卵清蛋白,鸡蛋(45,000)
谷氨酸脱氢酶,牛肝(55,000)
白蛋白,牛血清(66,000)
磷酸化酶B,兔肌肉(97,000)
β-半乳糖苷,大肠杆菌 (116000)
肌球蛋白,猪心脏(200,000)
通过考马斯蓝染色可产生12个蛋白质条带。

重悬

用100 μl去离子水进行溶解,可得到含有2-3.5mg蛋白质/ml的62mM Tris-HCl(pH6.8),1mM EDTA,4%蔗糖,10mM TCEP,2%SDS和0.01%溴酚蓝的溶液。

法律信息

SigmaMarker is a trademark of Sigma-Aldrich Co. LLC

分析证书

原产地证书 (CofO)

Mehtap Çelik et al.
Journal of food science and technology, 56(6), 3023-3032 (2019-06-18)
The aims of this research were to examine the effect of pH on extraction of proteins from sour cherry (Prunus cerasus L.) kernels, and to investigate the functional properties of the resulting protein concentrate. The optimum pH values for the...
Thermal denaturation of pea globulins (Pisum sativum L.)-molecular interactions leading to heat-induced protein aggregation.
Mession JL, et al.
Journal of Agricultural and Food Chemistry, 61, 1196-1204 (2013)
François Potin et al.
Journal of food science, 84(12), 3682-3690 (2019-11-07)
The purpose of this paper was to study the extraction conditions of hemp proteins from undelipidated press-cakes. The effects of different hydration conditions on protein recovery yield and polypeptide profile were evaluated: pH (2 to 12), ionic strength (0 to...
Sule Keskin et al.
Journal of economic entomology, 111(3), 1454-1460 (2018-03-13)
The objective of this study was to investigate the effects of storage and granary weevil, Sitophilus granarius (L.; Coleoptera: Curculionidae), infestation on pH, protein solubility (PS) and gel electrophoresis properties of meal and roller-milled flours of hard (Ceyhan-99 cv.) and...
Pavel Mokrejs et al.
Journal of cosmetic dermatology, 16(4), e21-e27 (2017-02-07)
Although keratin hydrolysates have become established as standard components in hair and nail cosmetics, studies on the moisturizing effects of keratin hydrolysates do not appear among contemporary literature. To test if adding keratin hydrolysate into an ointment base increases hydration...

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