S9200

Sigma-Aldrich

Anti-SORL1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:
Anti-LDLR class A repeats containing, Anti-SorLA-1, Anti-LR11, Anti-gp250, Anti-LRP9, Anti-Sortilin-related receptor, Anti-SORLA
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~250 kDa

species reactivity

human, rat

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

application(s)

western blot: 1-2 μg/mL using rat cerebellum (S1 fraction)

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... SORL1(6653)
mouse ... Sorl1(20660)
rat ... Sorl1(300652)

General description

Sortilin related receptor 1 (SORL1), a multifunctional protein, is also known as SorLA-1, LR11, LRP9 and gp250. It is a type-I transmembrane protein highly expressed in neurons. This protein belongs to the low-density lipoprotein receptor family. It contains a transmembrane domain, a large extracellular domain and a short cytoplasmic tail. SORL1 gene is located on human chromosome 11q 23/24. This mosaic receptor contains a domain that shares similarity with the vacuolar protein sorting 10 (VPS10) and with the low-density lipoprotein receptor (LDLR) family of proteins.

Specificity

Anti-SORL1 (C-terminal) specifically recognizes rat SORL1.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Anti-SORL1 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Sortilin related receptor 1 (SORL1) protein plays a key role in intracellular protein transport and endocytosis, cell signaling and the proliferation and migration of smooth muscle cells. It acts as a receptor for lipoproteins, glial cell-line-derived neurotrophic factor (GDNF) and platelet-derived growth factor (PDGF). Expression of this protein is developmentally regulated, with high transcript levels found during morphogenesis. SORLA is involved in development of atherosclerosis and in Alzheimer′s disease (AD). It mediates the uptake of apolipoprotein E (ApoE)-rich lipoproteins and is markedly induced in atheroma. SORL1 is known to play a regulating role in trafficking and processing of the amyloid precursor protein (APP) in neuronal cells. In the Golgi, SorLA/LR11 binding of APP leads to sequestration of APP and thereby it blocks the processing to the beta-amyloid peptide.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Vanessa Schmidt et al.
Cellular and molecular life sciences : CMLS, 74(8), 1475-1483 (2016-11-11)
Sorting-related receptor with A-type repeats (SORLA) is an intracellular sorting receptor that directs cargo proteins, such as kinases, phosphatases, and signaling receptors, to their correct location within the cell. The activity of SORLA assures proper function of cells and tissues...
Coding mutations in SORL 1 and A lzheimer disease
Vardarajan BN, et al.
Annals of Neurology, 77(2), 215-227 (2015)
Satoru Suwa et al.
International heart journal, 61(3), 470-475 (2020-05-01)
Cardiovascular events still occur despite statin-based lipid-lowering therapy in patients with coronary artery disease (CAD). LR11, a member of the low-density lipoprotein receptor family, is a novel marker for the proliferation of intimal smooth muscle cells, which are critical to...
L Jacobsen et al.
The Journal of biological chemistry, 271(49), 31379-31383 (1996-12-06)
The 39-40-kDa receptor-associated protein (RAP) binds to the members of the low density lipoprotein receptor gene family and functions as a specialized endoplasmic reticulum/Golgi chaperone. Using RAP affinity chromatography, we have purified a novel approximately 250-kDa brain protein and isolated...
Badri N Vardarajan et al.
Annals of neurology, 77(2), 215-227 (2014-11-11)
Common single nucleotide polymorphisms in the SORL1 gene have been associated with late onset Alzheimer disease (LOAD), but causal variants have not been fully characterized nor has the mechanism been established. The study was undertaken to identify functional SORL1 mutations...

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