Merck

SAB3700036

Sigma-Aldrich

Anti-Bovine IgG (H+L), F(ab′)2 fragment-Peroxidase antibody produced in rabbit

affinity isolated antibody, lyophilized powder

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别名:
HRP
NACRES:
NA.46

生物来源

rabbit

偶联物

peroxidase conjugate

抗体形式

affinity isolated antibody

antibody product type

secondary antibodies

克隆

polyclonal

形式

lyophilized powder

species reactivity

bovine

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

运输

wet ice

储存温度

2-8°C

target post-translational modification

unmodified

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此商品
SAB3700248SAB3700941SAB3700885
technique(s)

immunohistochemistry: suitable, western blot: suitable, indirect ELISA: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

chicken

biological source

goat

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

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一般描述

IgG (immunoglobulin G) contributes to 10−20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50kDa and 25kDa respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains. The variable region of IgG antibody is specific to antigens and is highly conserved. IgG antibody is abundantly present in mother′s milk.

特异性

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Bovine IgG and Bovine Serum. No reaction was observed against Anti-Pepsin or Anti-Rabbit IgG F(c).

免疫原

Bovine IgG whole molecule

生化/生理作用

IgG (immunoglobulin G) antibody provides protection against bacterial, fungal and viral infections. Maternal IgG is transferred to fetus through the placenta that is vital for the immune defense of the neonate against infections. IgG antibody has its function similar to IgM antibody in complement system activation. IgG is involved in hypersensitivity type II and type III. It helps in opsonization, complement fixation and cell-mediated cytotoxicity.

物理属性

Antibody format: IgG F(ab′)2

外形

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

重悬

Reconstitute with 500 μ;L deionized water (or equivalent).

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

Exclamation mark

警示用语:

Warning

危险声明

预防措施声明

危险分类

Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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IgG subclasses and allotypes: from structure to effector functions
Vidarsson G, et al.
Frontiers in Immunology, 5(4), 520-520 (2014)
Natural autoantibodies
Antonio C, et al.
Current Opinion in Immunology, 7(6), 812-818 (1995)
Placental transfer of IgG subclasses in a Japanese population.
Hashira S, et al.
Pediatrics International, 42(4) (2000)
Gestur Vidarsson et al.
Frontiers in immunology, 5, 520-520 (2014-11-05)
Of the five immunoglobulin isotypes, immunoglobulin G (IgG) is most abundant in human serum. The four subclasses, IgG1, IgG2, IgG3, and IgG4, which are highly conserved, differ in their constant region, particularly in their hinges and upper CH2 domains. These
Specific IgG for cat allergens in patients with allergic conjunctivitis.
Miyama A, et al.
International Ophthalmology, 35(4), 575-586 (2015)

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