SAB4200232

Anti-GAD65 antibody , Mouse monoclonal

clone N-GAD65, purified from hybridoma cell culture

• Synonym(s): Anti-GAD2, Anti-glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Anti-glutamate decarboxylase 65 kDa isoform
• MDL编号: MFCD03455043
• NACRES: NA.41

Properties

• 生物来源: mouse
• 质量水平: 200
• 偶联物: unconjugated
• 抗体形式: purified from hybridoma cell culture
• antibody product type: primary antibodies
• 克隆: N-GAD65, monoclonal
• 形式: buffered aqueous solution
• 分子量: antigen ~65 kDa
• species reactivity: human, mouse, monkey, rat
• 浓度: ~1.0 mg/mL
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.25-0.5 μg/mL using whole extracts of rat brain
• 同位素/亚型: IgG1
• UniProt登记号: Q05329
• 运输: dry ice
• 储存温度: −20°C
• Gene Information: human ... GAD2(2572) ; mouse ... Gad2(14417) ; rat ... Gad2(24380)

Description

一般描述

Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.

应用

Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.

包装

25, 200 μL

生化/生理作用

Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(F): Not applicable
• 闪点(C): Not applicable