Merck

SAB4200360

Sigma-Aldrich

Anti-Cyclophilin B antibody, Mouse monoclonal

enhanced validation

clone CYPB-3, purified from hybridoma cell culture

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别名:
Monoclonal Anti-CYP-S1, Monoclonal Anti-CYPB, Monoclonal Anti-OI9, Monoclonal Anti-PPIB, Monoclonal Anti-SCYLP, Monoclonal Anti-peptidylprolyl isomerase B
NACRES:
NA.41

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

antibody product type

primary antibodies

克隆

CYPB-3, monoclonal

形式

buffered aqueous solution

分子量

antigen ~20 kDa

species reactivity

human, mouse, rat

增强验证

independent
Learn more about Antibody Enhanced Validation

浓度

~1.0 mg/mL

technique(s)

western blot: 2.5-5.0 μg/mL using whole cell extracts of human HepG2 or rat NRK cells.

同位素/亚型

IgG1

UniProt登记号

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

Gene Information

human ... PPIB(5479)
mouse ... Ppib(19035)
rat ... Ppib(64367)

一般描述

Cyclophilin B belongs to the cyclophilin family of immunophilins. It is present in the cytoplasm, nucleus, endoplasmic reticulum (ER)/golgi and plasma membrane. The protein can also be secreted from cells. The associated gene is mapped to human chromosome 15q22.
Monoclonal Anti-Cyclophilin B (mouse IgG1 isotype) is derived from the hybridoma CYPB-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human Cyclophilin B, conjugated to keyhole limpet hemocyanin (KLH). Cyclophilin B (CypB) is a 21 kDa protein encoded by the peptidylprolyl isomerase B (PPIB) gene that belongs to the cyclophilin family, is a highly conserved class of proteins originally identified as cellular binding proteins for the immunosuppressive drug cyclosporin A. CypB is mainly located within the endoplasmic reticulum (ER) of all cell types.

免疫原

synthetic peptide corresponding to the C-terminal region of human Cyclophilin B, conjugated to KLH. The corresponding sequence is identical in mouse, monkey and canine and differs by one amino acid in rat Cyclophilin B.

应用

Monoclonal Anti-Cyclophilin B antibody produced in mouse has been used in immunoblotting and immunofluorescence.

生化/生理作用

Cyclophilin B (CypB) is involved in inflammation, viral infection and cancer. It also plays an important role in protecting cells against endoplasmic reticulum (ER) stress via its PPIase activity. Mutations in the PPIB gene give rise to recessive forms of osteogenesis imperfecta and knockdown of this gene decreases cell growth, proliferation and migration.
In endoplasmic reticulum (ER), Cyclophilin B works as a chaperone for exported proteins. It also participates in the intracellular transport of collagen and proper formation of collagen fibril. Cyclophilin B can bind to the Gag protein of human immunodeficiency type 1 (HIV-1) and helps in HIV replication. It is also involved in the infection of hepatitis C virus (HCV), Japanese encephalitis virus (JEV) and human papillomavirus type 16 (HPV16). Cyclophilin B protects against ER-mediated hypoxia-induced cell death. It is overexpressed in various malignancies.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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