Merck
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SAB4200641

Sigma-Aldrich

Monoclonal Anti-Brg1 antibody produced in rat

clone 3G4, purified from hybridoma cell culture

Synonym(s):
Anti-ATP-dependent helicase SMARCA4, Anti-BAF190, Anti-BAF190A, Anti-BRG1, Anti-BRG1-associated factor 190A, Anti-BRM/SWI2-related gene 1, Anti-MRD16, Anti-RTPS2, Anti-SMARCA4, Anti-SNF2, Anti-SNF2-beta, Anti-SNF2-like 4, Anti-SNF2L4, Anti-SNF2LB, Anti-SWI/SNF related, Anti-SWI2, Anti-actin dependent regulator of chromatin, Anti-brahma protein-like 1, Anti-global transcription activator homologous sequence, Anti-hSNF2b, Anti-homeotic gene regulator, Anti-matrix associated, Anti-member 4, Anti-mitotic growth and transcription activator, Anti-nuclear protein GRB1, Anti-protein BRG-1, Anti-protein brahma homolog 1, Anti-subfamily a, Anti-sucrose nonfermenting-like 4, Anti-transcription activator BRG1
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

3G4, monoclonal

形式

buffered aqueous solution

分子量

~185 kDa

species reactivity

mouse, human

浓度

~1 mg/mL

technique(s)

flow cytometry: 2.5-5 μg/test using HeLa cells
immunoblotting: 0.1-0.2.0 μg/mL using HeLa nuclear extract
immunofluorescence: 10 μg/mL using HeLa cells

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

Gene Information

human ... BRG1(6597)
mouse ... Brg1(20586)

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This Item
SAB4200676SAB1402802WH0054766M7
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

3G4, monoclonal

clone

10B4, monoclonal

clone

1G8, monoclonal

clone

1A6, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

~185 kDa

mol wt

monomer ~7 kDa, ~34 kDa (multimer)

mol wt

antigen ~42.28 kDa

mol wt

-

species reactivity

mouse, human

species reactivity

rat, mouse

species reactivity

human

species reactivity

human

一般描述

Monoclonal Anti-Brg1 (rat IgG1 isotype) is derived from the hybridoma 3G4 produced by the fusion of mouse myeloma cells (SP2) and lymph node cells from rat immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human Brm-related gene-1 (Brg1) protein. Brg1 or SWI/SNF (SWItch/Sucrose non-fermentable) related, matrix-associated, actin-dependent, regulator of chromatin, subfamily A, member 4 (SMARCA4) gene with 35 exons, is encoded by the gene mapped to human chromosome 19p13.

免疫原

a synthetic peptide corresponding to a sequence at the C-terminal region of human Brg1 protein

应用

Monoclonal Anti-Brg1 antibody produced in rat has been used in:
  • immunoblotting (~185 kDa)
  • flow cytometry
  • immunofluorescence

生化/生理作用

Brm-related gene-1 (Brg1) (or hBrm) protein is the central catalytic ATPase of the SWI/SNF chromatin-remodeling complex, which is involved in transcriptional modulation of hormone-responsive promoters through binding of the complex to various nuclear receptors and its recruitment to gene-specific promoters. In addition, BRG1 has also been shown to act as a transcriptional repressor. It interacts with retinoblastoma tumor suppressor to form a repressor complex which inhibits cell cycle proteins such as cyclins A, D1 and E. BRG1 participates in the firing of origins and the process of replication elongation. This function of BRG1 also facilitates embryogenesis. In addition, It functions as a tumor suppressor and maintains genome stability and prevent cancer.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


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