Merck

SAB4200683

Sigma-Aldrich

Anti-Human IgG4 antibody, Mouse monoclonal

clone HP-6025, purified from hybridoma cell culture

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别名:
Monoclonal Anti-Human IgG4 antibody produced in mouse
NACRES:
NA.46

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

HP-6025, monoclonal

形式

buffered aqueous solution

species reactivity

human

浓度

~1 mg/mL

technique(s)

immunoblotting: suitable
indirect ELISA: 0.15-0.3 μg/mL using 1 μg/ml human IgG4 myeloma proteins for coating.

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

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此商品
SAB4200713I7385SAB4200770
antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

clone

HP-6025, monoclonal

clone

GG-5, monoclonal

clone

HP-6025, monoclonal

clone

HP-6025, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

lyophilized powder

species reactivity

human

species reactivity

human

species reactivity

-

species reactivity

human

concentration

~1 mg/mL

concentration

~1 mg/mL

concentration

-

concentration

~2 mg/mL

一般描述

Monoclonal Anti-Human IgG4 (mouse IgG1 isotype) is derived from the HP-6025 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgG4 is a subclass of IgG with γ4 heavy chain.

特异性

Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.

免疫原

Purified human IgG4 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

应用

The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.

生化/生理作用

IgG2 and IgG4 deficiency is associated with IgA deficiency as found in patients of ataxia telangiectasia. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and thus may assist in the diagnosis of various disorders. IgG subclass expression pattern in malaria patients reveals positive association of IgG4 levels and malaria antigens but not with patients age.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

闪点(F)

Not applicable

闪点(C)

Not applicable


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其他客户在看

Slide 1 of 1

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Antibody structure, instability, and formulation.
Wang W
Journal of Pharmaceutical Sciences, 96, 1-26 (2007)

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