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Merck

SAB4200738

Sigma-Aldrich

Anti-FITC antibody, Mouse monoclonal

clone FL-D6, purified from hybridoma cell culture

别名:

Anti-Fluorescein isothiocyanate

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

品質等級

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

FL-D6, monoclonal

形狀

buffered aqueous solution

濃度

~1.0 mg/mL

技術

immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC
immunohistochemistry: suitable
indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC

同型

IgG1

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

Anti-FITC antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the FL-D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with FITC (fluorescein isothiocyanate)-BSA conjugate. This antibody specifically recognizes both free and conjugated FITC.
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light (with peak wavelength of approximately 495 nm) causing molecules to become excited and emit a visible yellow-green light (with peak wavelength of approximately 519 nm).

特異性

Monoclonal Anti-FITC antibody specifically recognizes both free and conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate).

免疫原

FITC-BSA conjugate

應用

Anti-FITC antibody, Mouse monoclonal may be used in:
  • immunoblotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry

生化/生理作用

FITC (fluorescein isothiocyanate) is a commonly used protein marker including antibodies in immunofluorescent techniques. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well-defined hapten.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

儲存和穩定性

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Discard working dilution samples if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析证书(COA)

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