Merck
All Photos(2)

SAB5600204

Sigma-Aldrich

Anti-beta-Actin antibody, Rabbit monoclonal

recombinant, expressed in HEK 293 cells, clone RM112, purified immunoglobulin

Synonym(s):
ACTB, Actin cytoplastic 1, actin beta, beta-actin

重组

expressed in HEK 293 cells

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

RM112, monoclonal
recombinant monoclonal

形式

buffered aqueous glycerol solution

species reactivity

all

technique(s)

ChIP: 1:200
ELISA: suitable (assay dependent concentration)
flow cytometry: suitable (assay dependent concentration)
immunocytochemistry: 1:200
immunohistochemistry: 1:200
immunoprecipitation (IP): 1:200
western blot: 1:1000

同位素/亚型

IgG

NCBI登记号

UniProt登记号

运输

wet ice

储存温度

−20°C

Gene Information

human ... ACTB(60)

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This Item
SAB5500001A2066SAB4502543
recombinant

expressed in HEK 293 cells

recombinant

expressed in proprietary host

recombinant

-

recombinant

-

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

RM112, monoclonal, recombinant monoclonal

clone

SP124, monoclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous solution

form

buffered aqueous solution

species reactivity

all

species reactivity

human (tested)

species reactivity

wide range, vertebrates, human, slime mold, amoeba, chicken

species reactivity

rat, human, mouse

technique(s)

ChIP: 1:200, ELISA: suitable (assay dependent concentration), flow cytometry: suitable (assay dependent concentration), immunocytochemistry: 1:200, immunohistochemistry: 1:200, immunoprecipitation (IP): 1:200, western blot: 1:1000

technique(s)

flow cytometry: 1:100, immunoblotting: 1:200, immunohistochemistry: 1:200

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25 using human or animal tissue sections, indirect immunofluorescence: 1:40 using chicken fibroblasts, western blot: 1:100 using chicken gizzard extract

technique(s)

ELISA: 1:20000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

特异性

This antibody reacts to beta-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin.

免疫原

A peptide corresponding to the N-terminus of beta-actin

外形

Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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