Merck
All Photos(4)

SAB5600274

Sigma-Aldrich

Anti-Phosphotyrosine antibody, Rabbit monoclonal

recombinant, expressed in HEK 293 cells, clone RM111, purified immunoglobulin

Synonym(s):
p-Tyr, tyrosine-phosphorylated proteins

重组

expressed in HEK 293 cells

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

RM111, monoclonal
recombinant monoclonal

形式

buffered aqueous glycerol solution

species reactivity

all

technique(s)

ChIP: 1:200-1:1000
ELISA: suitable (assay dependent concentration)
flow cytometry: suitable (assay dependent concentration)
immunocytochemistry: 1:200-1:500
immunohistochemistry: 1:200—1:500
immunoprecipitation (IP): 1:200-1:1000
western blot: 1:1000-1:5000

同位素/亚型

IgG

运输

wet ice

储存温度

−20°C

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This Item
SAB5600203P1869P5872
recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

-

recombinant

-

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

RM111, monoclonal, recombinant monoclonal

clone

RM102, monoclonal, recombinant monoclonal

clone

pT-154, monoclonal

clone

PT-66, monoclonal

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous solution

species reactivity

all

species reactivity

all

species reactivity

-

species reactivity

-

technique(s)

ChIP: 1:200-1:1000, ELISA: suitable (assay dependent concentration), flow cytometry: suitable (assay dependent concentration), immunocytochemistry: 1:200-1:500, immunohistochemistry: 1:200—1:500, immunoprecipitation (IP): 1:200-1:1000, western blot: 1:1000-1:5000

technique(s)

ChIP: 1:100-1:500, ELISA: suitable (assay dependent concentration), flow cytometry: suitable (assay dependent concentration), immunocytochemistry: 1:100-1:500, immunohistochemistry: 1:100-1:500, immunoprecipitation (IP): 1:100-1:500, western blot: 1:500 —1:2000

technique(s)

direct ELISA: suitable, immunohistochemistry: suitable, microarray: suitable, western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1.0 μg/mL using phosphotyrosine conjugated to BSA, radioimmunoassay: suitable, western blot: 0.25-0.5 μg/mL using total cell extract of human platelets

特异性

This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine.

免疫原

Phosphotyrosine-BSA conjugate

外形

Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable


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