Merck

SAE0045

Sigma-Aldrich

HRV-3C 蛋白酶

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

登录to View Organizational & Contract Pricing

Select a Size

别名:
Levlfqgp 站点蛋白酶, 人鼻病毒 3C 蛋白酶, 预断蛋白酶
NACRES:
NA.54

质量水平

检测方案

≥90% (SDS-PAGE)

形式

aqueous solution

分子量

21 kDa

浓度

0.8-1.2 mg/mL

运输

dry ice

储存温度

−20°C

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
SAE0067SAE0101PIC0007
HRV-3C Protease. N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

Sigma-Aldrich

SAE0045

HRV-3C 蛋白酶

SUMO Protease His tagged recombinant protein, lyophilized powder

Sigma-Aldrich

SAE0067

SUMO蛋白酶

form

aqueous solution

form

lyophilized powder

form

aqueous solution

form

ready-to-use solution

mol wt

21 kDa

mol wt

27 kDa

mol wt

27 kDa

mol wt

-

concentration

0.8-1.2 mg/mL

concentration

-

concentration

≥25,000 units/mL

concentration

-

shipped in

dry ice

shipped in

ambient

shipped in

dry ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

一般描述

来自人鼻病毒 14 类型的 HRV-3C 蛋白酶是一种在 8 个残基识别序列内特异性切割的蛋白酶。
蛋白水解裂解发生在 Gln 和 Gly 残基之间。
HRV-3C 蛋白酶是一种有用的工具,用于切割在载体结构域和目的蛋白之间以该序列作为融合蛋白表达的重组蛋白。
该重组版本含有 6 个组氨酸标签,可通过 IMAC 色谱法轻松去除。

应用

HRV-3C 蛋白酶已被用于去除 DsCystatin 蛋白上的 GST 标签。

单位定义

一单位HRV-3C蛋白酶定义为:在0°C条件下,在反应缓冲液中每小时消化1 nM底物肽(H-Glu-Ala-Leu-Phe-Gln-pNA)所需的酶量。反应缓冲液组成为25 mM HEPES(pH 7.5)、150 mM NaCl、1 mM EDTA和1 mM DTT。

外形

以溶液形式提供,含 50 mM Tris HCl(pH 7.5)、0.15 MNaCl、1 mM TCEP 和 50% (V/V) 甘油。

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

其他客户在看

Slide 1 of 3

1 of 3

Sigma-Aldrich

Sigma-Aldrich

SRP3032

肠激酶 人

Grad-seq analysis of Enterococcus faecalis and Enterococcus faecium provides a global view of RNA and protein complexes in these two opportunistic pathogens.
Michaux, et al.
microLife, 4, uqac027-uqac027 (2023)
An immunosuppressive tick salivary gland protein DsCystatin interferes with Toll-like receptor signaling by downregulating TRAF6
Ta S et al.9
Frontiers in Immunology, 9 (2018)
Zhengmao Xu et al.
Parasites & vectors, 12(1), 341-341 (2019-07-13)
Rhipicephalus haemaphysaloides is a widespread tick species in China and other South East Asian countries, where it is the vector of many pathogens. The objective of this study was to study the role of serpin (serine protease inhibitor) during the
Activity of the human rhinovirus 3C protease studied in various buffers, additives and detergents solutions for recombinant protein production
Raheem U et al.
PLoS ONE (2016)
Micah Rapp et al.
Cell reports, 35(1), 108950-108950 (2021-04-02)
Antibodies with heavy chains that derive from the VH1-2 gene constitute some of the most potent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-neutralizing antibodies yet identified. To provide insight into whether these genetic similarities inform common modes of recognition, we

商品

Tag Removal Proteases for Recombinant Protein Purification

Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门