Merck
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SAE0083

Sigma-Aldrich

Auresine

recombinant, lyophilized powder

EC索引号:
3.4.24.75

重组

expressed in Pichia pastoris

形式

lyophilized powder

specific activity

>500 units/mg protein

分子量

14.4 kDa

储存条件

room temperature (at +4 °C (in Storage Buffer))

储存温度

2-8°C

一般描述

Auresine is a portion of the zinc metalloprotease Staphylococcus aureus. The enzyme has glycyl-glycine endopeptidase activity and it specifically cleaves polyglycine crosslinks in the cellular wall of Staphylococcus species, including various strains of methicillin-resistant Staphylococcus aureus (MRSA). Auresine is thus useful for lysis of MRSA.

应用

Auresine can be used for isolation of protoplasts, nucleic acids, proteins, lipids and other components of Gram-positive bacterial cells. Auresine is particularly useful to lyse MRSA.. Thanks to its unique activity in non-physiological conditions (low conductivity and low temperatures), isolated components after auresine treatment, like nucleic acids and proteins, are not prone to degradation by released cellular enzymes.

单位定义

One unit will reduce the turbidity (A600) of suspension of S. aureus cells from 0.250 to 0.125 in 10 minutes in 50 mM glycine, pH 8.0 at 25°C in 6.0 mL reaction mixture.

制备说明

Auresine in soluble in water (20 mg/ml), yielding a clear solution. The product is active in 100 nM concentration (1.4 ug/mL) in low conductivity buffers (= 2 mS/cm) at temp. 0-40°C.

储存及稳定性

Auresine lyophilized powder should be stored at room temperature. Once dissolved in storage buffer, Auresine is stable at room temperature up to 4 months, at 4°C, -20°C, -80°C at least 2 years. Storage buffer: 20 mM Tris-HCl, pH 7.0, 200 mM NaCl, 10% glycerol.

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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