所有图片(1)

SAE0092

Sigma-Aldrich

变溶菌素 来源于球孢链霉菌 ATCC 21553

free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)

CAS号:
MDL编号:

形式

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

分子量

23 kDa
~18.5 kDa

特点

DNA free

运输

wet ice

储存温度

−20°C

应用

Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。

生化/生理作用

变溶菌素是一种 N-乙酰胞壁质酶。如溶菌酶一样,它是一种可裂解细菌细胞壁聚合物肽聚糖多糖的 β-N-乙酰胞壁酰-(1→4)-N-乙酰葡糖胺键的胞壁质水解酶。其羧基末端部分与识别和结合唯一的细胞壁聚合物有关。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌,如乳杆菌属和乳球菌属。

单位定义

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

外形

Lyophilized powder containing Ficoll® and sodium succinate buffer salts

法律信息

Ficoll is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls...
K Yokogawa et al.
Antimicrobial agents and chemotherapy, 6(2), 156-165 (1974-08-01)
Mutanolysin partially purified from the culture filtrate of Streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near pH 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. Mutanolysin exhibited maximal...
Rebecca M Corrigan et al.
PLoS pathogens, 7(9), e1002217-e1002217 (2011-09-13)
The cell wall is a vital and multi-functional part of bacterial cells. For Staphylococcus aureus, an important human bacterial pathogen, surface proteins and cell wall polymers are essential for adhesion, colonization and during the infection process. One such cell wall...
Martin Bastian Pedersen et al.
Journal of bacteriology, 190(14), 4903-4911 (2008-05-20)
Lactococcus lactis is a widely used food bacterium mainly characterized for its fermentation metabolism. However, this species undergoes a metabolic shift to respiration when heme is added to an aerobic medium. Respiration results in markedly improved biomass and survival compared...
Stacey M Broomall et al.
Applied and environmental microbiology, 82(2), 596-607 (2015-11-15)
Effective microbial forensic analysis of materials used in a potential biological attack requires robust methods of morphological and genetic characterization of the attack materials in order to enable the attribution of the materials to potential sources and to exclude other...

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