MISSION® shRNA Human Gene Family Set, DNA

Ubiquitin Hydrolases (DUBS)








Gene Information

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Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.


Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.
Number of Genes: 124, Number of Clones: 818
For a detailed listing of other available gene family sets, visit the gene family set website.


Use of this product is subject to one or more license agreements. For details, please see .
MISSION is a registered trademark of Sigma-Aldrich Co. LLC


12 - Non Combustible Liquids


Not applicable


Not applicable


Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


原产地证书 (CofO)

Benjamin Nicholson et al.
Future oncology (London, England), 3(2), 191-199 (2007-03-27)
Tagging proteins with mono- or poly-ubiquitin is now recognized as a multifaceted and universal means of regulating cell growth and physiology. It does so by controlling the cellular lifetime of nearly all eukaryotic proteins and the cellular localization of many...
Susan M Millard et al.
The Journal of cell biology, 173(4), 463-468 (2006-05-17)
Ubiquitylation is a key regulator of protein trafficking, and much about the functions of ubiquitin ligases, which add ubiquitin to substrates in this regulation, has recently come to light. However, a clear understanding of ubiquitin-dependent protein localization cannot be achieved...