Merck

SHC001

Sigma-Aldrich

MISSION®pLKO.1-puro空载体对照质粒 DNA

Contains no shRNA insert

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别名:
MISSION® 对照载体
MDL编号:
NACRES:
NA.51

质量水平

产品线

MISSION®

浓度

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

运输

dry ice

储存温度

−20°C

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此商品
SHC201SHC005SHC003
concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

一般描述

MISSION pLKO.1-puro 对照载体是一种慢病毒质粒载体。该载体不含 shRNA 插入,在使用 MISSION shRNA 文库克隆的实验中用作阴性对照。 这让人们可以检测转染对基因表达的影响,并解读 shRNA 克隆看到的敲低效应。氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外,通过与相容的包装质粒共转染,可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒。普尔科。1-puro 对照载体以 10μ 提供g 质粒 DNA,溶于 Tris-EDTA (TE) 缓冲液中,浓度为 500 ng/μl。

应用

MISSION® pLKO.1-puro 空载体对照质粒 DNA 已被用于合成和克隆 shRNA(靶向鼠分化簇 36 (CD36)),以生成慢病毒表达载体。
想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna

法律信息

使用本产品需遵守一个或多个许可协议。有关详细信息,请参阅http://sigmaaldrich.com/missionlicense
MISSION is a registered trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


Certificates of Analysis (COA)

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Cardiospecific CD36 suppression by lentivirus-mediated RNA interference prevents cardiac hypertrophy and systolic dysfunction in high-fat-diet induced obese mice.
Zhang Y
Cardiovascular Diabetology, 14 (2015)
AMP-activated protein kinase (AMPK)-induced preconditioning in primary cortical neurons involves activation of MCL-1.
Anilkumar U
Journal of Neurochemistry, 124 (2013)
Gregory J Baker et al.
Cancer research, 74(18), 5079-5090 (2014-07-20)
Natural killer (NK) cells safeguard against early tumor formation by destroying transformed target cells in a process referred to as NK immune surveillance. However, the immune escape mechanisms used by malignant brain tumors to subvert this innate type of immune
Timothy Andrew Couttas et al.
Metabolites, 10(6) (2020-06-12)
The number, position, and configuration of double bonds in lipids affect membrane fluidity and the recruitment of signaling proteins. Studies on mammalian sphingolipids have focused on those with a saturated sphinganine or mono-unsaturated sphingosine long chain base. Using high-resolution liquid
Irene Rodríguez-Sánchez et al.
PLoS pathogens, 15(1), e1007569-e1007569 (2019-01-25)
Human Cytomegalovirus (HCMV) infection induces several metabolic activities that are essential for viral replication. Despite the important role that this metabolic modulation plays during infection, the viral mechanisms involved are largely unclear. We find that the HCMV UL38 protein is

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shRNA Process and Diagram

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

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