Merck
All Photos(1)

SHC001

Sigma-Aldrich

MISSION®pLKO.1-puro空载体对照质粒 DNA

Contains no shRNA insert

Synonym(s):
MISSION® 对照载体
MDL编号:
NACRES:
NA.51

质量水平

200

产品线

MISSION®

浓度

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

运输

dry ice

储存温度

−20°C

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一般描述

MISSION pLKO.1-puro 对照载体是一种慢病毒质粒载体。该载体不含 shRNA 插入,在使用 MISSION shRNA 文库克隆的实验中用作阴性对照。 这让人们可以检测转染对基因表达的影响,并解读 shRNA 克隆看到的敲低效应。氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外,通过与相容的包装质粒共转染,可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒。普尔科。1-puro 对照载体以 10μ 提供g 质粒 DNA,溶于 Tris-EDTA (TE) 缓冲液中,浓度为 500 ng/μl。

应用

MISSION® pLKO.1-puro 空载体对照质粒 DNA 已被用于合成和克隆 shRNA(靶向鼠分化簇 36 (CD36)),以生成慢病毒表达载体。
想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna

法律信息

使用本产品须遵守一项或多项许可证协议。详情请参阅 http://sigmaaldrich.com/missionlicense
MISSION is a registered trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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