Merck
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SHC003

Sigma-Aldrich

MISSION® pLKO.1-puro-CMV-TurboGFP 阳性对照质粒 DNA

Green fluorescent protein marker to monitor transduction efficiency

Synonym(s):
MISSION® 对照载体
MDL编号:
NACRES:
NA.51

质量水平

200

产品线

MISSION®

浓度

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

运输

dry ice

储存温度

−20°C

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一般描述

MISSION ® TurboGFP 对照载体是一个 8347 碱基对的慢病毒质粒载体,含有编码 TurboGFP 的基因,由 CMV 启动子控制。TurboGFP 对照载体在使用 MISSION ® shRNA 文库克隆的实验中作为阳性转染/转导对照十分有用。 TurboGFP 是从桡足类 Pontellina plumata 中克隆的绿色荧光蛋白 copGFP 的改良变体。

氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外,通过与相容的包装质粒共转染,可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒。TurboGFP 对照载体以 10μg 质粒 DNA,溶于 Tris-EDTA (TE) 缓冲液中,浓度为 500 ng/μl.

应用

MISSION® pLKO.1-puro-CMV-TurboGFP 阳性对照质粒 DNA 已被用于构建 GATA2 过表达载体 (oeG2),方法是在 5′ 端去掉该载体的 GFP 盒。NheI 和 3′PstI 克隆位点,并用全长 GATA2 转录序列替代。
想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna

法律信息

使用本产品须遵守一项或多项许可证协议。详情请参阅 http://sigmaaldrich.com/missionlicense
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
TurboBeads is a trademark of TurboBeads LLC
TurboGFP is a trademark of Evrogen Co.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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