Merck
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SHC003V

Sigma-Aldrich

MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Transduction Particles

Green fluorescent protein marker to monitor transduction efficiency

Synonym(s):
MISSION® TurboGFP® Control Transduction Particles
NACRES:
NA.51

质量水平

200

产品线

MISSION®

浓度

≥1x106 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

运输

dry ice

储存温度

−70°C

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一般描述

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from the copepoda Pontellina plumata. The TurboGFP transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP (SHC003). It is a positive control to monitor transduction efficiency.

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

应用

MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.

法律信息

MISSION is a registered trademark of Sigma-Aldrich Co. LLC
TurboBeads is a trademark of TurboBeads LLC
TurboGFP is a trademark of Evrogen Co.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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