Merck
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SHC005

Sigma-Aldrich

MISSION® pLKO.1-puro eGFP shRNA对照质粒DNA

shRNA sequence targeting eGFP

Synonym(s):
MISSION® 对照载体
MDL编号:
NACRES:
NA.51

质量水平

200

产品线

MISSION®

浓度

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

运输

dry ice

储存温度

−20°C

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一般描述

MISSION® eGFP shRNA对照载体是含有7,091个碱基对的慢病毒质粒载体,其含有靶向eGFP的shRNA序列(GenBank登记号pEGFP U55761)。 在使用表达eGFP的细胞系的实验中,eGFP shRNA对照载体可用作阳性敲低对照。
氨苄青霉素和嘌呤霉素抗生素抗性基因分别适用于细菌或哺乳动物细胞的筛选。此外,通过与相容包装质粒 MISSION® 慢病毒包装混合物(产品编号SHP001)共转染,可以在包装细胞(HEK293T)中产生自灭活的复制功能不全的病毒颗粒。eGFP shRNA对照载体产品为10 μg质粒DNA,溶剂为Tris-EDTA (TE)缓冲液,浓度为500 ng/μl。

应用

想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna

法律信息

使用本产品须遵守一项或多项许可证协议。详情请参阅 http://sigmaaldrich.com/missionlicense
MISSION is a registered trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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