Merck
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SHC016V

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles

Targets no known genes from any species

Synonym(s):
MISSION® TurboGFP® Control Transduction Particles, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
NACRES:
NA.51

质量水平

200

产品线

MISSION®

浓度

≥1x106 VP/ml (via p24 assay)

运输

dry ice

储存温度

−70°C

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一般描述

The MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 μL at 1 x 106 TU/mL via p24 assay.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

应用

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines.

分析说明

To see more application data, protocols, vector maps visit sigma.com/shrna.

法律信息

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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