Merck
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SHC216

Sigma-Aldrich

MISSION® 对照载体

Targets no known genes from any species

Synonym(s):
MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
MDL编号:
NACRES:
NA.51

质量水平

200

产品线

MISSION®

浓度

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

运输

dry ice

储存温度

−20°C

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一般描述

The MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

应用

To see more application data, protocols, vector maps visit sigma.com/shrna.

法律信息

使用本产品须遵守一项或多项许可证协议。详情请参阅 http://sigmaaldrich.com/missionlicense
MISSION is a registered trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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