Merck
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SRE0001

Sigma-Aldrich

胃蛋白酶 来源于猪胃粘膜

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥3200 units/mg protein

别名:
胃液素 来源于猪胃, 胃蛋白酶 A
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

质量水平

400

形式

lyophilized powder

specific activity

≥3200 units/mg protein

分子量

35 kDa

杂质

salt, essentially free

颜色

white to off-white

UniProt登记号

application(s)

diagnostic assay manufacturing

运输

wet ice

储存温度

−20°C

Gene Information

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相关类别

包装

5, 10, 100 g in glass bottle

应用

胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

生化/生理作用

优先裂解:P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键

单位定义

一个单位将在 37°C、pH2.0 下每分钟产生 ΔA 280 0.001,以 TCA 可溶产物计,以血红蛋白为底物。(最终体积 = 16 mL。)

分析说明

最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。
E1%/280法测定蛋白

其他说明

查看有关 胃蛋白酶 的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

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原产地证书 (CofO)

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更多文件

Quotes and Ordering

Joseph S Fruton
The Quarterly review of biology, 77(2), 127-147 (2002-07-02)
Studies on gastric digestion during 1820-1840 led to the discovery of pepsin as the agent which, in the presence of stomach acid, causes the dissolution of nutrients such as meat or coagulated egg white. Soon afterward it was shown that
J Tack
Alimentary pharmacology & therapeutics, 22 Suppl 1, 48-54 (2005-07-27)
Gastro-oesophageal reflux disease is defined as the presence of symptoms or lesions that can be attributed to the reflux of gastric contents into the oesophagus. Aspiration and prolonged monitoring studies in humans have shown that reflux of gastric contents is
Shenlin Wang et al.
The Journal of biological chemistry, 289(2), 697-707 (2013-11-23)
Multidomain protein folding is often more complex than a two-state process, which leads to the spontaneous folding of the native state. Pepsin, a zymogen-derived enzyme, without its prosegment (PS), is irreversibly denatured and folds to a thermodynamically stable, non-native conformation
Yi Sun et al.
Journal of natural products, 77(7), 1749-1752 (2014-06-25)
Two linear peptides, ahpatinin Ac (1) and ahpatinin Pr (2), were isolated together with the known ahpatinin (i)Bu, pepstatin Ac, pepstatin Pr, and pepsinostreptin from a Streptomyces sp. derived from a deep-sea sediment. The structure of ahpatinin Pr (2) was
Tina L Samuels et al.
The Annals of otology, rhinology, and laryngology, 119(3), 203-208 (2010-04-16)
Diagnosis of extraesophageal reflux (EER) currently relies on tools designed for diagnosis of gastroesophageal reflux. Such tools lack the sensitivity and reproducibility to detect the less frequent and mildly acidic reflux associated with upper airway disease. Pepsin has been posited

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