Merck
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SRE0065

Sigma-Aldrich

HEPES溶液,1M

Reagent designed and manufactured under cGMP controls suitable for use in an IVD application

Synonym(s):
HEPES缓冲溶液, N-(2-羟乙基)哌嗪N′-(2-乙磺酸)

质量水平

形式

liquid

浓度

1 M

颜色

colorless

pH值(酸碱度)

7.2-7.5 (25 °C)

application(s)

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

包装

100, 500 mL
1 L

应用

HEPES 缓冲液保持正常的生理 pH 环境,通常用作细胞培养应用中的缓冲液。HEPES 缓冲液对细胞没有营养方面的益处,但是为细胞培养增加了额外的缓冲能力,特别是在二氧化碳培养箱外进行的实验期间。
该 1 M 储备溶液包含用氢氧化钠调节至 pH 7.35 的 HEPES。将浓缩缓冲液添加到细胞培养基中以达到所需浓度(通常为 10–25 mM)。

注:如果浓缩缓冲液中存在沉淀,则将瓶温和地加热至 37°C,混合直至完全溶解后稀释。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

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