Merck

T1503

Sigma-Aldrich

Trizma ®

Primary Standard and Buffer, ≥99.9% (titration), crystalline

别名:
2-氨基-2-(羟甲基)-1,3-丙二醇, THAM, Tris 碱, 三羟甲基氨基甲烷, 氨基丁三醇
线性分子式:
NH2C(CH2OH)3
CAS号:
分子量:
121.14
Beilstein:
741883
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

质量水平

描述

aminopeptidase substrate

检测方案

≥99.9% (titration)

形式

crystalline

pH值(酸碱度)

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

溶解性

methanol: soluble 26 mg/mL at 25 °C
ethylene glycol: soluble 79.1 mg/mL at 25 °C
water: soluble (678 g/l at 20 °C)

吸光度

≤0.05 at 290 nm at 40%

application(s)

diagnostic assay manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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此商品
T6066T4661T6791
Trizma® base Primary Standard and Buffer, ≥99.9% (titration), crystalline

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T1503

Trizma ®

Trizma® base BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

Sigma-Aldrich

T6066

Trizma ®

Trizma® base ≥99.9% (titration), crystalline

Sigma-Aldrich

T4661

Trizma ®

Trizma® base BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

Sigma-Aldrich

T6791

Trizma ®

form

crystalline

form

crystalline powder

form

crystalline

form

crystalline

pH

10.5-12

pH

10.0-11.5

pH

10.0-11.5

pH

10.5-12.0 (1 M in H2O)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

solubility

methanol: soluble 26 mg/mL at 25 °C, ethylene glycol: soluble 79.1 mg/mL at 25 °C, water: soluble (678 g/l at 20 °C)

solubility

water: soluble (666 mg/ml, yielding a clear colorless solution)

solubility

water: soluble (400 g plus 600 mL of water)

solubility

H2O: 1 M, colorless

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

一般描述

40% (w/w) 的水溶液是透明无色的。
Tris是用于生物化学和分子生物学公认的基础标准品和缓冲液。它可以单独用作缓冲液或混合缓冲液配方的组分,如Tris-EDTA(TE)缓冲液、Tris-乙酸盐-EDTA(TAE)缓冲液、Tris-硼酸盐-EDTA(TBE)缓冲液等。 它具有高纯度、较稳定、相对不吸湿、并且具有高的当量。

应用

Trizma®碱已用于:
  • 用作H缓冲液(细胞解离缓冲液)组分
  • 洗涤和浸润双夹心ELISA免疫酶技术测定反应孔
  • 用作重悬提取并烘干的蛋白质样品的测定缓冲液
  • 制备用于稳定蛋白质的Tris-HCl缓冲液
  • 用作从块茎中提取类胡萝卜素的缓冲液
  • 用作蛋白质印迹前蛋白提取过程的样品缓冲液组分
  • 用作十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE)的样品缓冲液组分
  • 制备用于磷酸钙(CaP)吸附测定的模拟体液(SBF)
  • 用作在不锈钢(SS)基材上沉积聚多巴胺(PDA)的缓冲液

其他说明

利用 Trizma规格表轻松对Trizma产品的规格进行比较。
所有缓冲液的 pH 值都取决于温度和浓度。Tris 缓冲液,温度每降低 1°C,pH 值增加约 0.03 个单位,每稀释十倍 pH 值降低0.03-0.05 个单位。
对于精确的应用,使用经过精心校准的 pH 计和玻璃/甘汞组合电极。

法律信息

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


Certificates of Analysis (COA)

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在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例 T1503).

示例

T1503
货号
-
25G
包装规格/数量

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PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

请输入1.000309185)

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Osteoprotective action of low-salt diet requires myeloid cell?derived NFAT5
Schroder A, et al.
JCI insight, 4(23) (2019)
F Foudrinier et al.
Journal of clinical microbiology, 41(4), 1681-1686 (2003-04-12)
The clinical value of immunoenzymatic (enzyme-linked immunosorbent assay) detection of anti-Toxoplasma immunoglobulin E (IgE) was assessed by studying 2,036 sera from 792 subjects, comprising seronegative controls and subjects with acute, active, reactivated, or congenital toxoplasmosis. Included were nonimmunized adults; pregnant
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XXX: Measurements of plasma concentrations of the incretin hormone GLP-1 are complex because of extensive molecular heterogeneity. This is partly due to a varying and incompletely known degree of C-terminal amidation. Given that virtually all GLP-1 assays rely on a

实验方案

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Enzymatic Assay of Carboxypeptidase

Objective: To standardize a procedure for the assay of Carboxypeptidase A

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To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

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