Merck

T1763

Sigma-Aldrich

Trypsin Agarose

buffered aqueous suspension, from bovine pancreas trypsin

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MDL编号:
eCl@ss:
42020142
NACRES:
NA.54

生物来源

bovine pancreas (trypsin)

质量水平

形式

buffered aqueous suspension

浓度

≥15 units/mL (packed gel)

标记范围

≥15 units per mL packed gel

基质

cross-linked beaded agarose

运输

wet ice

储存温度

2-8°C

相关类别

一般描述

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.

应用

A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography.
Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides.

其他说明

Insolubilized

单位定义

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

外形

Suspension in approx. 10 mM acetic acid, pH 3.2

象形图

Exclamation mark

警示用语:

Warning

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

WGK

WGK 3

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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