Merck
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T2601

SAFC

StableCell™胰蛋白酶溶液

1X, sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

All Photos(2)
Synonym(s):
胰蛋白酶-EDTA 溶液
MDL编号:
MFCD00130286

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Sigma-Aldrich

Sigma-Aldrich

T4049

胰蛋白酶-EDTA 溶液

0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

Sigma-Aldrich

Sigma-Aldrich

T3924

胰蛋白酶-EDTA 溶液

1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

StableCell™ Trypsin Solution 5X, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

SAFC

T2605

StableCell™胰蛋白酶溶液

5X, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

StableCell™ Trypsin Solution 10X, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride

SAFC

T2610

StableCell™胰蛋白酶溶液

10X, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride

Sigma-Aldrich

Sigma-Aldrich

T4174

胰蛋白酶-EDTA 溶液

10 ×, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride

Sigma-Aldrich

Sigma-Aldrich

T4299

胰蛋白酶-EDTA 溶液

1 ×, sterile-filtered, BioReagent, suitable for cell culture, 500 BAEE units porcine trypsin and 180 μg EDTA, 4Na per ml in Dulbecco′s PBS without calcium and magnesium

Accutase® solution sterile-filtered, suitable for cell culture

Sigma-Aldrich

A6964

Accutase® 溶液

sterile-filtered, suitable for cell culture

Trypsin-EDTA, In Hank′s Balanced Salt Solution, 0.25% Trypsin and 1mM EDTA, without Ca2+ and Mg2+ The Trypsin-EDTA, In Hank′s Balanced Salt Solution, 0.25% Trypsin & 1mM EDTA, without Ca 2+ & Mg 2+ is available in a 100 mL format.

Sigma-Aldrich

SM-2003

胰蛋白酶-EDTA，在Hank′s平衡盐溶液中，0.25%胰蛋白酶和1mM EDTA，不含Ca2+ 和Mg2+

The Trypsin-EDTA, In Hank′s Balanced Salt Solution, 0.25% Trypsin & 1mM EDTA, without Ca 2+ & Mg 2+ is available in a 100 mL format.

Trypsin 0.25%, In Hank′s Balanced Salt Solution, without Ca2+ and Mg2+ The Trypsin 0.25%, In Hank′s Balanced Salt Solution, without Ca 2+ & Mg 2+ is available in a 100 mL format.

Sigma-Aldrich

SM-2001

Trypsin 0.25%, In Hank′s Balanced Salt Solution, without Ca2+ and Mg2+

The Trypsin 0.25%, In Hank′s Balanced Salt Solution, without Ca 2+ & Mg 2+ is available in a 100 mL format.

Sigma-Aldrich

Sigma-Aldrich

T3449

TrypZean® 溶液 1×

recombinant, expressed in corn, sterile-filtered

质量水平

500

sterility

sterile-filtered

产品线

BioReagent

浓度

1X

technique(s)

cell culture | mammalian: suitable

运输

wet ice

储存温度

2-8°C

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一般描述

胰蛋白酶由223个氨基酸残基的单链多肽组成，其通过从胰蛋白酶原去除N-末端六肽而产生（在Lys-Ile肽键处被切割）。氨基酸序列通过6个二硫键交联。这是胰蛋白酶、β-胰蛋白酶的天然形式。β-胰蛋白酶可以在Lys-Ser残基处发生自裂解，并产生α-胰蛋白酶。胰蛋白酶是丝氨酸蛋白酶家族的成员。

包装

100, 500 mL

应用

该产品的典型用途是从培养表面除去粘附细胞。从其基底上分离所需的胰蛋白酶浓度主要依赖于细胞类型和培养基已使用的时间。

生化/生理作用

胰蛋白酶切割赖氨酸和精氨酸残基的 C 末端肽段。 如果酸性残基位于切割位点的任意一侧，则该反应的水解速度减慢，并且如果脯氨酸残基位于切割位点的羧基侧，则水解停止。胰蛋白酶活性的最佳 pH 条件为7-9。胰蛋白酶还可以切割氨基酸合成衍生物的酯和酰胺键。将 乙二胺四乙酸（EDTA） 添加到胰蛋白酶溶液中作为螯合剂，用于中和钙离子和镁离子以防止其抑制胰蛋白酶活性。去除这些离子会增加酶活性。

特点和优势

StableCell™胰蛋白酶是一种用于细胞解离的温和型溶液。当储存在2-8°C时可维持酶活不变，而我们的研究表面当保留在室温下最长达7周后，酶活依然可以保留>90%！
  • 节省时间 - 无需反复冻融
  • 节省空间 - 储存在2-8°C，节省冷冻空间
  • 无需分装
  • 最适合于您的细胞 - 在GMP环境下生产

制备说明

采用过高的胰蛋白酶浓度孵育细胞较长时间会对细胞膜造成损伤并杀死细胞。将胰蛋白酶溶解或将其从浓缩液进行稀释应采用不含有Ca2+或Mg2+的缓冲盐溶液。

储存及稳定性

推荐在到货后保存在2-8°C。在稳定性研究中，数据表明当储存在37°C时，该产品可保留≥90%的活性最长8周。

法律信息

StableCell is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Product Information Sheet

Product Information Sheet - T2601

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