Merck
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T2610

SAFC

StableCell™胰蛋白酶溶液

10X, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride

All Photos(2)
Synonym(s):
胰蛋白酶-EDTA 溶液
MDL编号:
MFCD00130286

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T4174

胰蛋白酶-EDTA 溶液

10 ×, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride

StableCell™ Trypsin Solution 1X, sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

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T2601

StableCell™胰蛋白酶溶液

1X, sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

Sigma-Aldrich

Sigma-Aldrich

T4049

胰蛋白酶-EDTA 溶液

0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

StableCell™ Trypsin Solution 5X, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

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T2605

StableCell™胰蛋白酶溶液

5X, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

Sigma-Aldrich

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T3924

胰蛋白酶-EDTA 溶液

1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

Sigma-Aldrich

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T4549

胰蛋白酶 溶液 来源于猪胰腺

sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in 0.9% sodium chloride

Trypsin-EDTA, In Hank′s Balanced Salt Solution, 0.25% Trypsin and 1mM EDTA, without Ca2+ and Mg2+ The Trypsin-EDTA, In Hank′s Balanced Salt Solution, 0.25% Trypsin & 1mM EDTA, without Ca 2+ & Mg 2+ is available in a 100 mL format.

Sigma-Aldrich

SM-2003

胰蛋白酶-EDTA，在Hank′s平衡盐溶液中，0.25%胰蛋白酶和1mM EDTA，不含Ca2+ 和Mg2+

The Trypsin-EDTA, In Hank′s Balanced Salt Solution, 0.25% Trypsin & 1mM EDTA, without Ca 2+ & Mg 2+ is available in a 100 mL format.

Trypsin from porcine pancreas lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid

Sigma-Aldrich

T4799

胰蛋白酶 来源于猪胰腺

lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid

Sigma-Aldrich

Sigma-Aldrich

T4424

胰蛋白酶 溶液 来源于猪胰腺

1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red

vibrant-m

T2600000

胰蛋白酶

BRP, European Pharmacopoeia (EP) Reference Standard

质量水平

500

sterility

sterile-filtered

产品线

BioReagent

浓度

10X

technique(s)

cell culture | mammalian: suitable

运输

wet ice

储存温度

2-8°C

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Related Categories

包装

100, 500 mL

应用

该产品的典型用途是使贴壁细胞从培养表面脱落。使细胞从附着面脱落所需的胰蛋白酶浓度主要取决于细胞类型和培养阶段。

生化/生理作用

胰蛋白酶可切割赖氨酸和精氨酸残基的C端肽。如果酸性残基位于切割位点的两侧，则该反应的水解速度减慢，而如果脯氨酸残基位于切割位点的羧基侧，则不水解。胰蛋白酶活性的最佳pH为7-9。胰蛋白酶还可以切割氨基酸合成衍生物的酯键和酰胺键。将EDTA作为螯合剂添加到胰蛋白酶溶液中，可中和掩盖胰蛋白酶作用肽键的钙镁离子。去除这些离子可增加酶活性。

特点和优势

StableCell™ 胰蛋白酶是一种温和的细胞分离溶液。在2-8°C下保存时，酶的活性可得以保留，我们的研究表明在室温下放置7周，其活性甚至可以保留>90％！
  • 节省时间 - 无需反复冻融
  • 节省空间 - 储存在2-8°C，节省冷冻空间
  • 无需分装
  • 最适合于您的细胞 - 在GMP环境下生产

组分

胰蛋白酶由含有223个氨基酸残基的单链多肽组成，由胰蛋白酶原在Lys - lle肽键处切去N-端六肽而得。这些氨基酸序列通过6个二硫键交联。这就是胰蛋白酶（即β-胰蛋白酶）的天然形式。BETA-胰蛋白酶可自溶，在Lys-Ser残基裂解，产生α-胰蛋白酶。胰蛋白酶是丝氨酸蛋白酶家族的成员。

制备说明

用浓度过高的胰蛋白酶长时间孵育细胞会破坏细胞膜并杀死细胞。将胰蛋白酶溶解或将其从浓缩液进行稀释应采用不含有Ca2+或Mg2+的缓冲盐溶液。

储存及稳定性

到货后推荐的保存温度为2-8°C。在稳定性研究中，数据表明该产品在37°C下可保持≥90％的活性最多8周。

法律信息

StableCell is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Product Information Sheet - T2610

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