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Merck

T4549

Sigma-Aldrich

胰蛋白酶 溶液 来源于猪胰腺

sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in 0.9% sodium chloride

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.75

生物源

Porcine

品質等級

無菌

sterile-filtered

產品線

BioReagent

形狀

solution

分子量

23.4 kDa

技術

cell culture | mammalian: suitable

pH值

7.6

運輸包裝

dry ice

儲存溫度

−20°C

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應用

胰蛋白酶消化多肽时,胰蛋白酶:多肽使用约 1:100 至 1:20 的比例。该产品的典型用途是去除培养表面的贴壁细胞。使细胞脱离其底物所需的胰蛋白酶浓度主要取决于细胞类型和培养时间。胰蛋白酶也被用于细胞培养过程中的细胞重悬,蛋白质组学研究中的蛋白质消化和各种凝胶消化。其他的应用还包括通过基于膜的技术评估结晶,并在一项研究中确定蛋白质折叠速率和产量可能受到动力学陷阱存在的限制。

生化/生理作用

胰蛋白酶切割赖氨酸和精氨酸残基 C 末端的肽。如果酸性残基位于裂解位点两侧,则该反应的水解速率减慢,如果脯氨酸残基位于裂解位点的羧基侧,则水解停止。胰蛋白酶活性的最适 pH 为 7-9。胰蛋白酶也可裂解氨基酸合成衍生物的酯键和酰胺键。向胰蛋白酶溶液中加入 EDTA,作为螯合剂,可中和钙和镁离子,使胰蛋白酶作用的肽键模糊。去除这些离子会增加酶的活性。

丝氨酸蛋白酶抑制剂,包括 DFP、TLCK、APMSF、AEBSEF 和抑肽酶等,将抑制胰蛋白酶。

注意

本品应在-20℃ 冷冻保存。

單位定義

一个 BAEE 装置在 pH 7.6、25°C 条件下,使用 BAEE 作为底物,每分钟可产生 0.001 A253

準備報告

这是溶于 0.9% 氯化钠的 25 g/L 猪胰蛋白酶溶液。

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Autophagic (type II) cell death has been suggested to play pathogenetic roles in cerebral ischemia. Growth arrest and DNA damage response 45b (Gadd45b) has been shown to protect against rat brain ischemia injury through inhibiting apoptosis. However, the relationship between
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A clinically relevant risk factor for Clostridioides difficile-associated disease (CDAD) is recent antibiotic treatment. Although broad-spectrum antibiotics have been shown to disrupt the structure of the gut microbiota, some antibiotics appear to increase CDAD risk without being highly active against

实验方案

胰蛋白酶常用于从表面进行贴壁细胞的解离。我们可提供一系列不同的胰蛋白酶溶液来满足您的特定细胞系需求。

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

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