Merck
All Photos(7)

T6199

Sigma-Aldrich

抗-α-微管蛋白抗体,小鼠单克隆

enhanced validation

clone DM1A, purified from hybridoma cell culture

Synonym(s):
单克隆抗-α-Tubulin
NACRES:
NA.41

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

DM1A, monoclonal

形式

buffered aqueous solution

分子量

antigen ~50 kDa

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

包装

antibody small pack of 25 μL

增强验证

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

浓度

~1 mg/mL

technique(s)

immunocytochemistry: 0.5-1 μg/mL using cultured chicken fibroblasts (CFB)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total tissue extract from chicken gizzard

同位素/亚型

IgG1

UniProt登记号

application(s)

research pathology

运输

dry ice

储存温度

−20°C

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

1 of 4

This Item
T0198T7451T6074
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

clone

DM1A, monoclonal

clone

D66, monoclonal

clone

6-11B-1, monoclonal

clone

B-5-1-2, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen ~50 kDa

mol wt

antigen ~50 kDa

mol wt

-

mol wt

antigen ~50 kDa

一般描述

抗-α-微管蛋白抗体,小鼠单克隆(小鼠IgG1亚型)是由小鼠骨髓瘤细胞(NS1)和纯化的鸡脑微管蛋白免疫BALB/c小鼠脾细胞融合产生的DM1A杂交瘤衍生而来。该亚型采用双扩散免疫法并采用小鼠单克隆抗体同分型试剂(产品号为ISO2)测定。

微管蛋白是微管的主要组成部分。这种细胞内圆柱形丝状结构存在于几乎所有的真核细胞中。微管在细胞有丝分裂、胞内运输、鞭毛运动和细胞骨架中起着结构和活动的作用。微管蛋白是一个异源二聚体,包含α-微管蛋白和β-微管蛋白。两个亚基的相对分子质量都约为50 kDa,具有相当大的同源性。除了α-和β-微管蛋白外,其他几个微管蛋白已被确定,使各种微管蛋白类的数量达到7种。这些微管蛋白大多具有明显的亚细胞定位和一系列明显不同的功能。七种不同的微管蛋白家族中有四个成员是近期被鉴定得到的,包括δ, ξ, η和ε-微管蛋白。η和ε-微管蛋白是由数据库搜索得到。微管系统至少含有三种α-微管蛋白异构体。两种异构体由两个α-微管蛋白基因编码,这两个基因都是转录并编码极其相似的蛋白质。第三种异构体是通过翻译后修饰形成的。至少有三种微管蛋白亚基的修饰过程已得到描述:大脑中β-微管蛋白的磷酸化,脊椎动物组织中去除羧基末端酪氨酸形成的a-微管蛋白,以及α-微管蛋白中赖氨酸氨基的乙酰化。

α-微管蛋白,又称微管蛋白 α 4a (TUBA4A),定位于人类基因组2q35。该基因编码α-微管蛋白家族的一个成员,该成员含448个氨基酸。微管蛋白的α-亚基的分子量为50000。

识别α-微管蛋白的单克隆抗体与其他微管蛋白亚型的单克隆抗体一起(β,β-微管蛋白亚型I +II, β-微管蛋白亚型III,酪氨酸微管蛋白,α-微管蛋白的乙酰化形式)为研究微管蛋白在细胞内的分布以及细胞骨架的静态和动态方面提供了一种特异的且重要工具。

特异性

抗-α-微管蛋白抗体小鼠单克隆识别多种生物(如人类、牛、小鼠和鸡)中位于α-微管蛋白亚型(氨基酸426-430)C末端的表位。
该抗体在免疫印迹检测中对α-tubulin具有特异性,并可用于培养细胞或组织切片中α-tubulin的定位。该抗体可与鸡成纤维细胞产生最佳的反应。

免疫原

来自鸡胚脑的微管。

应用

抗-α-微管蛋白抗体,小鼠单克隆已用于免疫细胞荧光和蛋白印迹分析。

生化/生理作用

微管蛋白α4a(TUBA4A)突变与肌萎缩侧索硬化症(ALS)相关。

α-微管蛋白或α 4A(TUBA4A)在细胞生长和分裂中起重要作用。它还参与各种细胞过程,如细胞活力,信号发展和细胞形状的维持。TUBA4A基因突变与多种癌症的发生有关,如口腔癌、乳腺癌、直肠癌、肺癌和前列腺癌。此外,TUBA4A的变异可导致偶发性肌萎缩侧索硬化症(ALS)。

外形

溶液在0.01 M磷酸缓冲盐中,pH 7.4,含1%牛血清白蛋白和15 mM叠氮钠。

制备说明

储存及稳定性:存储在2-8°C,最长一个月。为长期保存,分装为工作溶液冷冻保存于-20°C。不建议反复冻融或存放在无霜冰柜中。如果长期储存时出现轻微浑浊,请在使用前通过离心澄清溶液。工作稀释液如果在12个小时内未使用,则应丢弃。

其他说明

为了使用各种技术和制剂获得最佳结果,我们建议通过滴定确定最佳工作稀释度。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物应用。

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service