Merck
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T6414

Sigma-Aldrich

胰蛋白酶抑制剂 来源于大豆

solution, sterile-filtered, suitable for, suitable for cell culture

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别名:
SBTI
CAS号:
9035-81-8
MDL编号:
MFCD00082103
NACRES:
NA.75

生物来源

Glycine max (soybean)

质量水平

100

无菌性

sterile-filtered

产品线

BioReagent

形式

solution

分子量

20,100 Da

浓度

10 mg/mL

technique(s)

cell culture | mammalian: suitable

溶解性

balanced salt solution: 1 mg/mL
concentrate: >10 mg/mL, hazy, amber-yellow
phosphate buffer: 10 mg/mL
water: 10 mg/mL
serum-free medium: soluble

适用性

suitable for

UniProt登记号

P01070

运输

dry ice

储存温度

−20°C

Gene Information

Glycine max (soybean) ... KTI3(547831)

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SRE0044T6522T2327
Trypsin inhibitor from Glycine max (soybean) solution, sterile-filtered, suitable for, suitable for cell culture

Sigma-Aldrich

T6414

胰蛋白酶抑制剂 来源于大豆

Trypsin Inhibitor from Glycine max (soybean) Suitable for manufacturing of diagnostic kits and reagents, suitable for cell culture

Sigma-Aldrich

SRE0044

胰蛋白酶抑制剂 来源于大豆

Trypsin inhibitor from Glycine max (soybean) powder, BioReagent, suitable for cell culture

Sigma-Aldrich

T6522

胰蛋白酶抑制剂 来源于大豆

Trypsin Inhibitor from Glycine max (soybean) BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

Sigma-Aldrich

T2327

胰蛋白酶抑制剂 来源于大豆

sterility

sterile-filtered

sterility

-

sterility

-

sterility

-

form

solution

form

lyophilized powder

form

powder

form

lyophilized powder

mol wt

20,100 Da

mol wt

14 kDa

mol wt

20.1 kDa

mol wt

-

concentration

10 mg/mL

concentration

-

concentration

10 mg/mL

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

应用

针对内皮细胞培养物传代进行了优化。
来源于大豆的胰蛋白酶抑制剂用于以下应用：
  • 分散的腺泡的过滤和接种
  • 磷酸化 tau 和免疫印迹分析的相对定量
  • VIL-4 和 IL-4 cDNA 的亚克隆化和转化，以及蛋白质表达和纯化（来源于大豆的胰蛋白酶抑制剂作为阳性对照）。

生化/生理作用

该抑制剂能够在较小程度上抑制胰蛋白酶、胰凝乳蛋白酶和纤溶酶。它还能抑制蛋白酶血浆激肽释放酶和凝血因子X，机制与抑制胰蛋白酶类似。胰蛋白酶抑制剂不会抑制金属蛋白酶、基于组织的激肽释放酶、酸性蛋白酶或硫代蛋白酶。该抑制剂通过与蛋白酶活性位点按1:1化学计量形成复合物，然后在抑制剂上切割单个精氨酸-异亮氨酸键。抑制作用是可逆的且具有pH依赖性。

其他说明

含有色谱纯化的大豆胰蛋白酶抑制剂和补铁小牛血清。
查看更多关于 胰蛋白酶抑制剂的信息。

单位定义

一个胰蛋白酶单位是指在25°C、pH 7.6条件下，以N-α-苯甲酰基-L-精氨酸乙酯（BAEE）为底物，每分钟产生0.001的 A253所需的酶量。

制备说明

在 Dulbecco′s 磷酸盐缓冲盐水中制备，不含钙和镁。
胰蛋白酶抑制剂能够以10 mg/mL溶于水和磷酸盐缓冲液。它能够以1 mg/mL溶于平衡盐溶液和无血清培养基中。浓度大于10 mg/mL的浓缩液可能浑浊，呈黄色至琥珀色。使用胰蛋白酶消化细胞后，重悬于胰蛋白酶抑制剂溶液中，使得用于解离的胰蛋白酶溶液浓度达到1 mg/mL。然后将细胞悬浮液以1000rpm离心，得到细胞沉淀。

在2-8℃下短期储存时，溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317 - H334

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells. EV lipid composition influences the fluidity and stability of EV membranes and reflects the availability
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实验方案

StableCell™胰蛋白酶溶液

StableCell™胰蛋白酶溶液设计用于执行标准胰蛋白酶溶液所实现的细胞解离，但不同的是前者无需等分、冷冻和解冻。这样可以节省大量时间。

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

Rat Dermal Fibroblasts (RDF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Astrocytes (HA) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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Chromatograms

application for HPLC

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