所有图片(1)

T6424

Sigma-Aldrich

胰蛋白酶 来源于人类胰腺

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

质量水平

200

形式

salt-free, lyophilized powder

包装

vial of ≥1,000 BAEE units

UniProt登记号

储存温度

−20°C

Gene Information

相关类别

一般描述

胰蛋白酶分子有两个结构域:一个与酶的活性位点和色氨酸残基有关;另一种与 8-苯胺萘-1-磺酸结合有关。
胰蛋白酶是一种丝氨酸蛋白酶。胰腺可产生前体胰蛋白酶原并将其分泌到肠中。肠粘膜中的肠激酶可将胰蛋白酶原转化为胰蛋白酶。

应用

胰蛋白酶已被用于一项评估戊型肝炎病毒和人类星状病毒之间的相似性的研究。在一项研究中也使用了胰蛋白酶来表征培养原代成人上皮祖细胞或干细胞的一种独特技术。

单位定义

使用BAEE作为底物,一个BAEE单元将在pH7.6,25℃下产生每分钟0.001的A253。 反应体积= 3.2mL(1cm光程)。

数量

至少 1,000 个 BAEE 单位/瓶

分析说明

通过 E1%/280 确定的蛋白质。

其他说明

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

Kelly A Dryden et al.
Journal of molecular biology, 422(5), 650-658 (2012-06-30)
Human astroviruses (HAstVs) are a major cause of gastroenteritis. HAstV assembles from the structural protein VP90 and undergoes a cascade of proteolytic cleavages. Cleavage to VP70 is required for release of immature particles from cells, and subsequent cleavage by trypsin
Industrial Enzymes and Their Applications (1998)
Cynthia Luz Marcelo et al.
BMC dermatology, 12, 8-8 (2012-06-26)
Primary keratinocytes derived from epidermis, oral mucosa, and urothelium are used in construction of cell based wound healing devices and in regenerative medicine. This study presents in vitro technology that rapidly expands keratinocytes in culture by growing monolayers under large
Janina Boyken et al.
Neuron, 78(2), 285-297 (2013-04-30)
Neurotransmission involves calcium-triggered fusion of docked synaptic vesicles at specialized presynaptic release sites. While many of the participating proteins have been identified, the molecular composition of these sites has not been characterized comprehensively. Here, we report a procedure to biochemically
Paul P Geurink et al.
Journal of medicinal chemistry, 56(3), 1262-1275 (2013-01-17)
Proteasomes degrade the majority of proteins in mammalian cells by a concerted action of three distinct pairs of active sites. The chymotrypsin-like sites are targets of antimyeloma agents bortezomib and carfilzomib. Inhibitors of the trypsin-like site sensitize multiple myeloma cells

实验方案

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).

相关内容

胰蛋白酶(EC 3.4.21.4)的酶学测定方案

本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯(BAEE)作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定(A253,光程= 1cm)方案:

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