所有图片(1)

T6522

Sigma-Aldrich

胰蛋白酶抑制剂 来源于大豆

powder, BioReagent, suitable for cell culture

别名:
SBTI
CAS号:
EC 号:
MDL编号:
NACRES:
NA.75

质量水平

300

生物来源

Glycine max (soybean)

类型

Type I-S

产品线

BioReagent

形式

powder

specific activity

(One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.)

分子量

20.1 kDa

浓度

10 mg/mL

应用

cell culture | mammalian: suitable

溶解性

balanced salt solution: 1 mg/mL
serum-free medium: soluble

运输

ambient

储存温度

2-8°C

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包装

25, 100, 5×100, 250, 500 mg in glass bottle
1, 5 g in glass bottle

应用

本品经过细胞培养应用测试。 胰蛋白酶已被用于评估来自弧菌属V26产毒素红树林分离株的碱性蛋白酶在动物细胞培养中的潜在应用。 胰蛋白酶也被用于改善 嗜酸性粘液中的真菌检测。

生化/生理作用

该抑制剂能够在较小程度上抑制胰蛋白酶、胰凝乳蛋白酶和纤溶酶。它还能抑制蛋白酶血浆激肽释放酶和凝血因子X,机制与抑制胰蛋白酶类似。胰蛋白酶抑制剂不会抑制金属蛋白酶、基于组织的激肽释放酶、酸性蛋白酶或硫代蛋白酶。该抑制剂通过与蛋白酶活性位点按1:1化学计量形成复合物,然后在抑制剂上切割单个精氨酸-异亮氨酸键。抑制作用是可逆的且具有pH依赖性。

组分

大豆胰蛋白酶抑制剂是一种单体蛋白质,含有181个氨基酸残基,是由两个二硫键连接而成的单个多肽链。

单位定义

一个胰蛋白酶单位是指在使用BAEE作为底物时,在25°C、 pH 7.6条件下每分钟产生0.001的ΔA253 所需的酶量;反应体积3.2 ml,1 cm光路。
一个胰蛋白酶单位是指在25°C、pH 7.6条件下,以N-α-苯甲酰基-L-精氨酸乙酯(BAEE)为底物,每分钟产生0.001的 A253所需的酶量。

制备说明

胰蛋白酶抑制剂能够以10 mg/mL溶于水和磷酸盐缓冲液。它能够以1 mg/mL溶于平衡盐溶液和无血清培养基中。浓度大于10 mg/mL的浓缩液可能浑浊,呈黄色至琥珀色。使用胰蛋白酶消化细胞后,重悬于胰蛋白酶抑制剂溶液中,使得用于解离的胰蛋白酶溶液浓度达到1 mg/mL。然后将细胞悬浮液以1000rpm离心,得到细胞沉淀。

在2-8℃下短期储存时,溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。

分析说明

1mg胰蛋白酶抑制剂将抑制1.0-3.0 mg胰蛋白酶,其中,胰蛋白酶活力约为10,000个BAEE单位/毫克蛋白质。

象形图

Health hazard

警示用语:

Danger

危险声明

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?   

    Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference:     J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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实验方案

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

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